高脂血、溶血标本对荧光定量聚合酶链反应测定低水平HBV-DNA的影响

摘要

Objective To investigate the influence of heperlipemia and hemolysis specimens on the fluorescence quantitative PCR determination results of low level HBV‐DNA .Methods The serum samples were collected from 100 hepatitis B patients with normal triacylglycerol (TG) (≤1 .46 mmol/L and (4 .0-9 .0) × 103 IU/mL by real time fluorescence quantitative PCR) in our hospital and performed the instant detection .In addition ,100 high TG samples of hepatitis B 5‐item negative and HBV‐DNA non‐detection and 100 whole blood samples of hepatitis B 5‐item nega‐tive and HBV‐DNA non‐detection were collected and performed the simultaneous HBV‐DNA detection of fluores‐cence quantitative PCR on the hyperlipemia and non‐hyperlipemia specimens ,hemolysis specimen and non‐hemolytic serum .And the paired t test was conducted .Results The HBV‐DNA detection levels had statistically significant differences between hyperlipemia and non‐hyperlipemia specimens and between hemolytic serum and non‐hemolytic serum (P<0 .05) .Conclusion The fluorescence quantitative PCR detection results of hyperlipemia and hemolysis specimens of low HBV‐DNA level are reduced compared with those of instant detection for fasting collection speci‐mens .%目的：探讨高脂血、溶血标本对荧光定量聚合酶链反应（PCR）测定低水平 HBV‐DNA检测结果的影响。方法对收治的100例三酰甘油（TG）正常（TG≤1．46 mmol／L）、实时荧光定量PCR测定为（4．0～9．0）×103 IU／mL的乙型肝炎患者采集血清标本，进行即刻检测，另外选择100份乙肝五项全阴且测不出HBV‐DNA的高TG标本和100份乙肝五项全阴且测不出 HBV‐DNA的全血标本分别制备成高脂血、溶血血清标本，同时进行高脂血和非高脂血、溶血血清和非溶血血清的 HBV‐DNA荧光定量PCR检测，并进行配对 t检验。结果高脂血和非高脂血、溶血血清和非溶血血清的 HBV‐DNA水平差异有统计学意义（P＜0．05）。结论高脂血、溶血标本低水平HBV‐DNA荧光定量PCR定量检测结果较空腹采集即刻检测的结果降低。