血清胸苷激酶1在儿童急性淋巴细胞白血病诊疗中的应用

摘要

Objective To study the clinical application of serum thymidine kinase 1 (sTK1) in the diagnosis and treatment of childhood acute lymphoblastic leukemia .Methods The Western blot‐enhanced chemiluminescence assay was adopted to detect serum TK1 level in 39 cases of childhood acute lymphoblastic leukemia patients before and after chemotherapy and contemporaneous 30 healthy children .Results The sTK1 level before chemotherapy in the patients group was higher than that in the complete remission group and the partial remission group after chemo‐therapy ,and the healthy control group ,the differences were statistically significant(P< 0 .01);the sTK1 level had statistical differences among different curative effect groups after chemotherapy(complete remission group ,partial re‐mission group and non‐remission group) (P<0 .01);the sTK1 level had no statistical difference between the non‐re‐mission group and before chemotherapy (P>0 .05);the positive detection rate of sTK1 before chemotherapy was sig‐nificantly higher than that after chemotherapy and the healthy control group(P<0 .01) .Conclusion The sTK1 de‐tection has the important reference value for screening ,assisted diagnosis and evaluation of chemotherapeutic effect in childhood acute lymphoblastic leukemia .%目的：探讨血清胸苷激酶1（sTK1）在儿童急性淋巴细胞白血病临床诊疗中的应用。方法采用免疫印迹增强化学发光法检测39例急性淋巴细胞白血病儿童患者化疗前、化疗后的sTK1浓度及30例同期健康体检儿童的sTK1浓度。结果患儿组化疗前的sTK1浓度明显高于化疗后完全缓解组、部分缓解组及对照组，差异有统计学意义（P＜0．01）；化疗后不同疗效组（完全缓解组、部分缓解组、未缓解组）之间sTK1浓度差异有统计学意义（P＜0．01）；未缓解组与化疗前的sTK1浓度差异无统计学意义（P＞0．05）；化疗前sTK1阳性检出率明显高于化疗后及对照组，差异有统计学意义（P＜0．01）。结论 sTK1的检测对于儿童急性淋巴细胞白血病的筛查、辅助诊断、化疗效果的评估具有重要参考价值。