重度溶血对人血清生化项目检测干扰及消除干扰影响的分析

摘要

Objective To analyze the interference of severe hemolysis on the detection results of human serum biochemical items and methods for eliminating the interference .Methods 150 venous blood samples in our hospital from January to December 2013 were selected .5 mL of venous blood was collected from the subject and equally divid‐ed into clean and dried test tubes :one as the observation group and another as the control group .The TOSHIBA TBA‐120 automatic biochemical analyzer was adopted to conduct the detection reagent by Beijing LEADMAN kit . The operation of instrument and kit were carried out according to the instructions and operating rules .The serum of the observation group was hemolyzed serum ,while which of control group was normal serum .The sample was centri‐fuged by the high‐speed centrifuge ,with the centrifugal time of 5 min and speed of 3 000 r/min .Then the same set of operating personnel operated the detection .The test items included the biochemical indicators of blood glucose ,alka‐line phosphatase ,alanine aminotransferase ,aspartate aminotransferase ,total cholesterol ,total bilirubin ,total protein , uric acid and albumin .Results The blood glucose and alkaline phosphatase results of the hemolytic samples in the observation group were lower than those of normal samples in the control group ;the alanine aminotransferase ,aspar‐tate aminotransferase ,total cholesterol ,total bilirubin ,uric acid ,and albumin results of hemolytic samples of in the observation group were higher than those of normal samples in the control group .Conclusion The hemolysis phe‐nomenon will influence the results of clinical serum test items ,which exerts the impact on the evaluation of doctor′s diagnosis and treatment effect .Should actively prevent hemolysis after collecting the blood samples for ensuring the accuracy of the serum detection results .%目的：分析重度溶血对人血清生化项目检测结果的干扰及消除干扰的方法。方法选取2013年1～12月钟山县人民医院静脉血样本150份，受检者抽取5 mL 静脉血液，将样本平分到洁净干燥试管中，一支为观察组，另一支为对照组。采用东芝 TBA‐120全自动生化分析仪，检测试剂采用北京利德曼试剂盒，仪器操作和试剂盒操作均按照说明书及操作规范进行。观察组样本为溶血血清，对照组为正常血清，采用高速离心机对样本进行离心处理，离心时间5 min ，转速为3000 r／min ，然后由同一组操作人员进行上机操作检测。检测项目包括血液中血糖、碱性磷酸酶、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、总蛋白、尿酸、清蛋白生化项目检测指标。结果观察组溶血样本的血糖、碱性磷酸酶的指标低于对照组样本；观察组溶血样本的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆固醇、总胆红素、尿酸、清蛋白的指标均高于对照组样本。结论溶血现象会影响临床血清检验项目的结果，并对医生诊断及治疗效果评价产生影响，在血液样本采集后应积极预防溶血现象，确保血清检测结果的准确性。