Sysmex XT-2000i全自动血细胞分析仪对不同类型胸腔积液中白细胞和红细胞检测的应用评价

摘要

目的：研究Sysmex XT‐2000i全自动血细胞分析仪对不同类型胸腔积液中白细胞和红细胞检测的可行性。方法使用Sysmex XT‐2000i全自动血细胞分析仪对246份不同类型的胸腔积液进行白细胞和红细胞计数，并对白细胞进行分类检测，同时与显微镜人工计数分类结果进行比对。结果在189例无分类不明细胞（团）的胸腔积液中，红细胞计数小于5000×106／L时，仪器计数高于手工计数结果，差异有统计学意义（ P＜0．05）；在57例有分类不明细胞（团）的胸腔积液中，红细胞计数小于10000×106／L时，仪器计数高于手工计数结果，差异有统计学意义（P＜0．05）。当胸腔积液标本中含有分类不明细胞（团）时，仪器对标本中白细胞计数结果高于手工计数结果，差异有统计学意义（P＜0．05），仪器未能对此类标本中的白细胞进行分类；当胸腔积液标本中无分类不明细胞（团）时，仪器对标本中白细胞计数结果与手工计数结果相比较，差异无统计学意义（ P＞0．05），但仪器仅对97例标本的白细胞进行分类，占51．32％（97／189）；且仪器可进行分类的标本，其单个核与多个核细胞分类与手工分类相比较，差异无统计学意义（P＞0．05），相关系数 r均大于0．97。结论 Sysmex XT‐2000i全自动血细胞分析仪对胸腔积液中红细胞和白细胞的计数与分类有一定的局限性，只适用于部分标本或项目，实际工作中可根据标本情况进行操作，并结合人工镜检进行报告。%Objective To study the feasibility of the Sysmex XT‐2000i automated hematology analyzer for de‐tecting while blood cells (WBC) and red blood cells(RBC) in different types of pleural effusion .Methods The Sys‐mex XT‐2000i automated hematology analyzer was used to perform the WBC count ,RBC count and WBC classifica‐tion in 246 different types of pleural effusion specimen ,meanwhile the detection results were compared with those de‐tected by using the manual microscopic counting method .Results In 189 pleural effusion samples without unknown classification of cell (group) ,when RBC content <5 000 × 106/L ,the instrument count was higher than the manual count ,with statistically significant difference (P<0 .05);in 57 pleural effusion specimens with unknown classification of cells (group) ,when RBC content <10 000 × 106/L ,the instrument count was higher than the manual count ,with statistically significant difference(P<0 .05) .When the pleural effusion samples with unknown classification of cells (group) ,the instrument WBC count was higher than the manual WBC count ,with statistically significant difference (P<0 .05) ,moreover the instrument fails to classify WBC in the samples ;in the pleural effusion samples without un‐known classification of cells (group) ,the instrument WBC count and the manual WBC count had no statistically sig‐nificant difference (P>0 .05) ,but only 97 pleural effusion samples were performed the WBC classification by instru‐ment ,accounting for 51 .32% (97/189);moreover for the samples classified by instrument ,the classification results of mononuclear and multiple nuclear cell by instrument and manual method had no statistically significant differences (P>0 .05) and the correlation coefficient was >0 .97 .Conclusion The Sysmex XT‐2000i automated hematology an‐alyzer for conducting the count of RBC and WBC and WBC classification in pleural effusion samples has certain limi‐tations and may be only suitable for partial samples or items ,in the practical work ,the operating should be conducted according to the sample condition and the results should be reported by combining with the manual microscopic exam‐ination .