酶联免疫吸附试验中影响全自动加样系统加样准确性的因素分析

摘要

Objective To investigate the influence of different volumes of sampling needle and liquid height on the accuracy and precision of the full‐automatic sampling system in ELISA tests .Methods The two sample amounts of 10 μL and 100 μL were selected and the each sample amount was divided into 4 groups for conducting the experi‐ment .The different volumes (400 μL and 800 μL) of sampling needle and different liquid heights (infiltration and non‐infiltratio) were used to conduct sampling .The final sampling amount ,mean relative error (E) and coefficient of variation (CV) were calculated in each group .Then the results were compared with the actual working standard .Re‐sults E of sampling 10 μL was -37 .9% - -2 .8% and CV was 3 .29% -14 .49% ;E of 100 μL was -1 .9% -0 .8% ,CV was 0 .44% -0 .95% ,respectively .Conclusion The different volumes of sampling needle and liquid height can affect the sampling amount of 10 μL obviously ,but less affect the sampling amount of 100μL .So the appropriate volume of sampling needle and liquid height should be selected to conduct the ELISA experiment when using the full‐automatic sampling device .%目的：探讨酶联免疫吸附试验（ELISA）中使用不同容量加样针和分液高度，对全自动加样系统加样准确度和精密度的影响。方法选取10μL和100μL两种加样量，每种加样量分4个小组进行实验，分别使用不同容量（400μL和800μL ）的加样针和不同分液高度（浸润和未浸润）加样，计算每组的最终加样量、均值相对误差（E）和变异系数（CV），与实际工作标准进行比较。结果10μL 加样 E在－37．9％～－2．8％，CV 在3．29％～14．49％；100μL加样 E在－1．9％～0．8％，CV在0．44％～0．95％。结论不同容量加样针和分液高度对10μL加样影响较为明显，对100μL加样影响较小，在使用全自动加样系统进行ELISA时，应当选择适当容量的加样针和分液高度。