某国产双向血培养瓶细菌培养分离效果的评价

摘要

目的 对某国产双向血培养瓶的灵敏度和抗菌药物吸附能力分析,并评价其细菌分离效果.方法 (1)灵敏度检测:用无菌生理盐水将标准菌株配制成0.5麦氏单位,倍比稀释形成浓度梯度后分别接种于该双向血培养瓶,常规培养.(2)抗菌药物吸附能力:用M H肉汤稀释头孢噻肟和庆大霉素,形成浓度梯度系列,分析铜绿假单胞菌对未经树脂吸附处理过和经过树脂吸附处理过的药物溶液M IC的变化.(3)150例临床标本同时用Bect/Alert 血培养瓶和该双向血培养瓶培养,并对其检出率、检出时间和检出种类进行统计.结果 (1)灵敏度:大肠埃希菌、葡萄球菌均为102CFU/mL,20 h检测出阳性;肺炎链球菌102CFU/mL,48 h检测出阳性,10 CFU/mL,72 h检测出阳性,流感嗜血杆菌102,CFU/mL 20 h检测出阳性;白假丝酵母菌10 CFU/mL,48 h检测出阳性;(2)抗菌药物吸附:每毫升树脂吸附头孢噻肟、庆大霉素分别为800.00、33.33 μg;(3)Bect/Alert 血培养瓶和该双向血培养瓶阳性检出率均为20.67%,都检出19种病原菌.结论 该双向血培养瓶灵敏度较高,抗菌药物吸附能力强,操作简单易行,价格便宜,适合基层医院.%Objective To analysis the sensitivity and adsorbability to antibiotic and to evaluate the effect of bacterial isolation of a domestic biphase blood(Pangtong)culture system.Methods Sensitivity:dilute 0.5 McF typical strains of ATCC with SPSS to form concentration gradient and then separately inoculate into the blood culture bottles,routine cultivate.Adsorbability:dilution cefotaxime and gentamicin with M H broth to form concentration gradient.Analysis the change of MIC of pseudomonas aeruginosa to two antibiotics with and without treated.Cultivate 150 clinic specimen with Bect/Alert and PangTong culture bottles,and compare the detection rate,time and category.Results T he sensitivity to Escherichia coli and Staphylococcus is 102 CFU/mL within 20 h,to Streptococcus pneumoniae is 102CFU/mL and 10 CFU/mL within 48 h and 72 h,re-spectively,to Haemophilus influenzae is 102CFU/mL within 20 h,to candida albicans is 102CFU/mL within 48 h.Adsorbability is 800.00 μg and 33.33 μg to cefotaxime and gentamicin per ml resin.The positive ratio of Bect/Alert and PangTong biphase blood culture bottles both is 20.67% without difference,and 19 pathogens were detectied.Conclusion The sensitivity of PangTong biphase blood culture bottle is high and adsorbability to antibiotic is powerful.There was no differce to Bect/Alert and PangTong biphase blood culture bottles to investigate clinic specimen,but PangTong biphase blood culture bottle need no special equipment.With simple manipulation and cheap,PangTong biphase blood culture bottle fits to primary hospital.