不同剂量无水乙醇致兔胃黏膜损伤模型的建立与评价

摘要

目的 观察不同剂量无水乙醇对兔胃黏膜的损伤程度.方法 将28只新西兰兔随机分为A、B、C、D组,每组7只,A组为对照组,B、C、D组分别以2.35、1、0.5 ml/kg无水乙醇灌胃造成胃黏膜损伤模型,从兔的一般状况、胃大体、胃病理学三方面来评价胃黏膜的损伤程度.结果与A组相比,B、C、D三组胃大体及病理组织切片观察均有胃黏膜的损伤.胃大体观察,B组明显可见溃疡；C组可见胃黏膜充血水肿及点状糜烂；D组胃黏膜表面未见明显损伤.组织切片观察可见B组损伤达肌层;C组损伤达黏膜层；D组损伤仅累及黏膜上皮.结论 无水乙醇可用于建立胃黏膜损伤模型,2.35 ml/kg无水乙醇灌胃可建立明显的胃溃疡模型；1 ml/kg无水乙醇灌 胃可建立糜烂出血性胃黏膜损伤模型；0.5 ml/kg无水乙醇灌胃胃黏膜损伤不明显.%Objective To observe the degree of injuries in rabbit gastric mucosal induced by different doses of anhydrous ethanol. Methods Twenty-eight New Zealand White rabbits were randomly divided into A, B, C, D groups (n=7). Group A was control group. The anhydrous ethanol 2.35 ml/kg , 1.00 ml/kg, 0.50 ml/kg with the dossage of were taken by intragastric administration to induce gastric mucosal lesion in group B, C, D respectively . The degree of gastric mucosal lesion was assessed by general morphology, biopsy and general physical condition. Results Compared with A group, gastric mucosal lesions were observed in the other three groups.Through general morphology of gastric, gastric ulcers were observed in group B, hyperemia, edema and erosion of gastric mucosal were observed in group C, but no significant lesion was observed in group D. Through biopsy, the lesions in group B were observed in muscle, mucosa in group C and nucosa epithelial in group D. Conclusion Anhydrous ethanol can induce gastric mucosal lesion models, 2.35ml/kg can induce gastric ulcer model, 1.00 ml/kg can induce gastric mucosal lesion model of erosion and hemorrhage, and 0.5ml/kg dosage did not show significant effects on gastric lesion.