裸鼹鼠与C57BL/6小鼠自噬调节的比较研究

摘要

Objective A comparative study between naked mole rat and C57BL/6 mouse on autophagy regulation is conducted,providing a reference for the research of mechanism in high autophagy,anti-cancer,slow aging of NMR.Methods Electron microscopy and western blotting are use to detect the activtity of autophagy in the liver and lung tissue of newborn naked mole rat and C57BL/6.Reagents interfere the autophagy level of fibroblasts derived from naked mole rat and mouse.The cell number is tested after 24,48,72 hours by CCK-8 kit.Meanwhile,expression of Beclin 1 at each time point is detected by western blotting.Results Compared with mice,the naked mole rat tissue have significantly higher LC3B expression.The NMR cells have a certain amount of resistance from 3-MA.Rapamycin's proliferation inhibition on NMR-S(fibroblasts derived from skin of naked mole rats)was significantly greater than that of mouse cells.Conclution NMR tissue possess a high autophagy,Cells from NMR have greater ability of self-regulation under extraneous autophagy inhibition,compared to C57BL/6.%目的 比较裸鼹鼠与小鼠的自噬调节,以期为裸鼹鼠高自噬、抗癌、衰老缓慢等生物学特性的研究提供参考.方法 利用电镜技术和蛋白印迹检测新生裸鼹鼠和C57BL/6小鼠肝脏和肺脏组织的自噬水平.自噬干扰裸鼹鼠成纤维细胞和小鼠成纤维细胞,24、48、72h CCK-8细胞计数法检测细胞的增殖情况,蛋白印迹检测各组细胞各个时间点Beclin 1的表达水平.结果 与C57BL/6小鼠比较,裸鼹鼠组织的LC3B表达显著较高.裸鼹鼠细胞对自噬抑制剂3-MA具有一定的耐药性,自噬诱导剂雷帕霉素对裸鼹鼠细胞的增殖抑制显著高于小鼠细胞.结论 裸鼹鼠组织具有高自噬水平,应对外界的自噬抑制压力时,裸鼹鼠细胞的自身调节能力明显高于C57BL/6小鼠.