非浓缩尿蛋白电泳在原发性高血压肾损伤中的应用评价

摘要

目的 探讨十二烷基磺酸钠-琼脂糖凝胶(Sodium dodecyl sulphat-agarose gel electrophoresis,SDS-AGE)非浓缩尿蛋白电泳在原发性高血压肾损伤中的诊断价值.方法 收集2016年3月～2016年8月在南京医科大学第一附属医院就诊的原发性高血压患者57例,根据尿蛋白定性结果进行分组,同时选取同期健康体检者20例作为健康对照组,进行SDS-AGE非浓缩尿蛋白电泳、24h尿蛋白(24h urine protein,24h Upro)、血清肌酐(serum creatinine,SCr)和尿素氮(blood urea nitrogen,BUN)水平测定,多样本总体均数间的比较采用LSD-t检验.结果 57例原发性高血压患者中见病理性蛋白尿30例(30/57,52.6％).尿蛋白(+/-)组见肾小管性蛋白尿2例(2/5,40％),尿蛋白(1+)组和尿蛋白(2+)组均见各种类型蛋白尿,尿蛋白(3+)组见肾小球性蛋白尿和混合性蛋白尿.在不同类型蛋白尿的24h Upro、SCr和BUN定量比较中发现,单纯白蛋白性、肾小球性、肾小管性和混合性这四种类型蛋白尿24h Upro的水平有明显差异,差异有统计学意义(P＜0.05).而SCr和BUN的检测只有混合性蛋白尿类型明显高于其余三种类型,差异有统计学意义(P＜0.05).结论 非浓缩尿蛋白电泳技术可直观反映尿蛋白组分,判断肾脏损伤部位,更早地提示原发性高血压肾功能的改变.%Objective To study the diagnostic value of SDS-AGE of non-concentrated proteinuria electrophoresis in essential hypertension renal injury.Methods 57 patients with essential hypertension were selected and divided according to the qualitation results of urine protein.SDS-AGE electrophoresis,24h Upro,SCr and BUN were performed and detected.LSD-t test was used for the analysis.Results In 57 cases essential hypertension patients,there were 30 cases of pothological proteinuria which includes 2 cases of tubular proteinuria in Pro (+/-)group,all types of proteinuria in Pro (+) and Pro (2 +) group,glomerular and mixed proteinuria in Pro (3 +)group.There were statistical significant differences between each group including simple,glomerular,tubular and mixed proteinuria in the levels of 24h Upro (P ＜ 0.05).Conclusion SDS-AGE of non-concentrated proteinuria electrophoresis can detect urinary protein components,and assess the position of kidney damage,which reflect the renal injury in essential hypertension earlier.