It was important to find a method for analyzing PCR products by automatic analysis station in clinical labs. A total of 208 clinical samples of Acinetobacter baumannii were examined by traditional PCR-agarose gel electrophoresis and PCR-Tag fluorescence, respectively. The results showed that two methods presented similar results in the sensitivity without statistical significance. PCR-Tag method showed low fluorescent background and high signal-to-noise (S/N) ratio. The procedure of PCR-Tag fluorescence seemed less DNA contaminated. The technology had a potential to be used as an instant detection method in clinical labs. It was suitable for the automatic sample analyzers in clinical labs.%建立一种自动化、高通量化的PCR产物鉴定方法并应用于临检实验室.对临床分离后经过生化反应鉴定的208例鲍曼不动杆菌,运用PCR-Tag(尾标)荧光技术进行检测分析,阳性率达到92％,与同等条件下所进行的普通引物PCR方法相比,结果无显著差异(P＞0.05).使用PCR-Tag荧光法检测鲍曼不动杆菌,可满足自动化、高通量、高可靠性的现代临床分子检测技术的要求,符合临床分子细菌学快速检测的发展方向.
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