不同胃黏膜病变组织中诱导型一氧化氮合酶mRNA的表达

摘要

Aim: To investigate the expression of inducible nitric oxide synthase(iNOS) mRNA in human gastric cari-noma tissue, precarcinoma changes, chronic inflammation tissue and to identify the significance of iNOS expression. Methods : iNOS mRNA were detected in 35 cases chronic superficial gastritis ( CSG) , 24 cases chronic atrophic gastritis with intestinal metaplasia( CAG + IM) , 21 cases gastric mucosal atypical hyperplasia( GMAH) and 65 cases gastric carcinoma ( GC) tissue specimens using in situ hybridization. Results: There was significant difference on positive expression rate of iNOS mRNA among GC (75.4% ,49/65) ,CSG(17. 1% ,6/35) , CAG + IM(45.8% ,11/24) and GMAH (52. 4% ,11/ 21 )tissues(x2 = 31.646,P <0.001 ). The expression of iNOS mRNA in GC was associated with histological grade, invasive depth and lymph node metastasis^2 =5. 122, 5. 182, and 13. 213 ,P < 0. 05) .without size, location (x2 =1.866, and 0. 366 ,P > 0.05 ) . Conclusion: iNOS might be closely correlated with occurrence, invasion and metastasis of GC.%目的:检测胃癌、癌前病变及慢性炎症胃黏膜组织中诱导型一氧化氮合酶(iNOS)mRNA的表达情况,探讨iNOS与胃癌发生发展的关系.方法:采用原位分子杂交法对35例慢性浅表性胃炎、24例慢性萎缩性胃炎伴肠化生、21例不典型增生及65例胃癌组织中iNOS mRNA表达进行检测.结果:iNOS mRNA在胃癌组织细胞中的表达明显增强,其阳性率(75.4%,49/65)与慢性浅表性胃炎(17.1%,6/35)、慢性萎缩性胃炎伴肠化生(45.8%,11/24)及不典型增生(52.4%,11/21)胃黏膜组织相比较,差异有统计学意义(χ2=31.646,P<0.001).胃癌组织中iNOS mRNA的阳性表达与组织学分型、浸润深度及有无淋巴结转移有关(χ2分别为5.122、5.182和13.213,P均<0.05),与肿瘤大小和肿瘤部位无关(χ2分别为1.866和0.366,P均>0.05).结论:iNOS可能参与了胃癌形成的全过程,不仅与胃癌形成有关,且与胃癌的侵袭及转移有关.