肺癌组织中Nrf2、Keap1和NQO1蛋白的表达

摘要

目的:研究肺癌组织中Nrf2、Keap1和NQO1蛋白表达的情况.方法:采用免疫组化法检测67例肺癌患者的肺癌组织及癌旁正常组织中Nrf2、Keap1和NQO1蛋白的表达,并分析Keap1、Nrf2和NQO1蛋白的表达与肺癌临床病理特征的关系.结果:Keap1蛋白在肺Ⅰ、Ⅱ、Ⅲ期鳞癌和Ⅲ期、Ⅳ期腺癌中的表达高于癌旁正常组织(P<0.05);Nrf2蛋白在肺Ⅰ期鳞癌和Ⅱ、Ⅲ、Ⅳ期腺癌中的表达低于癌旁正常组织(P<0.05);NQO1蛋白在肺Ⅰ期鳞癌中的表达低于癌旁正常组织(P<0.05),在Ⅱ期腺癌中的表达高于癌旁正常组织(P<0.05).3种蛋白的表达与肺癌患者的临床分期和是否侵及胸膜有关(P<0.05).结论:肺癌组织中Keap1、Nrf2、NQO1蛋白表达异常,提示Keap1-Nrf2/ARE通路在肺癌的发生发展中可能发挥重要的作用.%Aim;To study the protein expressions of Nrf2, Keapl and NQO1 in lung cancer tissue. Methods:Immuno-histochemistry was applied to detect the protein expressions of Keap 1, Nrf2 and NQO1 in 67 cases of lung cancer tissue and adjacent normal tissue. ReSUltS;The protein expression of Keapl was significantly higher in human phase Ⅰ , Ⅱ and Ⅲ lung squamous cell carcinoma and phase Ⅲ and IV lung adenocarcinoma than normal tissue (P < 0. 05). The protein expression of Nrf2 was significantly lower in human phase I lung squamous cell carcinoma and phase Ⅱ, Ⅲ and IV lung adenocarcinoma than normal tissue (P < 0. 05). The protein expression of NQO1 was significantly lower in human phase I lung squamous cell carcinoma and was significantly higher in human phase Ⅱ lung adenocarcinoma than normal tissue (P < 0. 05 ). The protein expressions of Keap 1, NQO1 and Nrf2 were related with clinical classification and distant metastasis ( P < 0.05). Conclusion;The abnormal expressions of Keapl , Nrf2,and NQO1 in lung cancer indicate that Keap 1 -Nr￡2/ARE pathway may play an important role in lung cancer initiation and promotion .