白藜芦醇对铅暴露小鼠学习记忆能力及氧化应激相关因子的影响

摘要

目的:探讨白藜芦醇对铅暴露小鼠学习记忆能力及氧化应激相关因子的影响.方法:将50只昆明小鼠随机分成5组,每组10只,即空白对照组、铅暴露组、白藜芦醇低、中、高剂量干预组.自由饮水形式染毒8周后,以Morris水迷宫实验测试小鼠学习记忆能力;用Z-5000型石墨炉原子吸收光谱仪测定血铅、海马组织铅含量;测定血清和海马组织中SOD活力和MDA含量.结果:Morris水迷宫实验结果显示,各组小鼠逃避潜伏期比较,差异有统计学意义(F=9.044,P＜0.001).各组小鼠血铅和海马组织铅含量比较,差异均有统计学意义(F=24.142和17.511,P均＜0.001);与铅暴露组相比,白藜芦醇干预组海马组织铅含量降低,差异有统计学意义(P<0.05).各组小鼠血清和海马组织SOD活力比较,差异均有统计学意义(F=35.190和11.933,P均＜0.001);各组小鼠血清和海马组织MDA含量比较,差异有统计学意义(F=5.610和4.406,P均<0.05).与空白对照组比较,铅暴露组和3个白藜芦醇干预组小鼠血清、海马组织SOD活力降低(P<0.05);与铅暴露组相比,白藜芦醇中剂量和高剂量干预组小鼠血清、海马组织SOD活力升高(P<0.05).结论:白藜芦醇干预可降低铅暴露小鼠血铅、海马组织铅含量,拮抗其对学习记忆能力的损伤作用,提高SOD活力、降低MDA含量.%Aim:To explore the effects of resveratrol (RES) on the learning memory performance and oxidative stress in lead exposure mice.Methods: Fifty Kunming mice were randomly divided into five groups , which were control group , lead exposure only group (Pb) , low ( L) , middle ( M) , and high ( H) dose RES interfering groups.The mice were allowed freely to drink water.After eight weeks feeding , Morris water maze (MWM) test was employed to evaluate the leam -ing memory ability of the mice.The lead levels in blood and hippocampus were analyzed by graphite furnace atomic absoip -tion spectrometry.Serum and hippocampal SOD activity and MDA level were measured .Results: MWM test showed that the escape latency among the 5 groups had significant differences (F=9.044 , P < 0.001 ).The lead levels in blood and hippocampus had significant differences (F = 24.142 and 17.511 ,P <0.001).Compared with Pb group ,the lead levels in hippocampus of RES interfering groups reduced ( P < 0.05 ).The SOD activity in blood and hippocampus showed signifi -cant differences among groups (F=35.190 and 11.933,P <0.001).The MDA levels also showed significant differences among groups (F=5.610 and 4.406,P <0.05).Compared with control group,the serum and hippocampal SOD activity significantly decreased in Pb group and the three RES interfering groups (P <0.05).Compared with Pb group,the SOD activity in M and H groups significantly increased (P < 0.05).Conclusion: RES could reduce the lead levels in blood and hippocampus , reverse the learning memory damage in lead exposure mice , as well as increase SOD activity and reduce the level of MDA.