充血性心力衰竭患者血清sICAM-1及白细胞膜黏附分子水平检测

摘要

目的:分析充血性心力衰竭(CHF)患者血清可溶性细胞间黏附分子-1(sICAM-1)及白细胞膜黏附分子(CD11a和CD18)水平的变化.方法:采集80例CHF患者和50例健康对照者血清标本,采用ELISA法进行血清sICAM-1检测,采用流式细胞仪检测CD11a和CD18.结果:CHF患者血清sICAM-1水平及CD11a、CD18阳性细胞百分率高于对照组(t分别为10.186、11.999和13.764,P均<0.05).不同基础心脏病CHF患者上述指标差异均无统计学意义(F=2.578、1.723、0.214,P均>0.05),不同心功能分级CHF患者间血清sICAM-1水平,CD11a、CD18阳性细胞百分率差异均有统计学意义(F=23.953、15.592和25.384,P<0.001),Ⅳ级患者高于Ⅱ、Ⅲ级患者(P<0.05).结论:ICAM-1介导的心脏炎症反应可能是诱发或加重CHF的原因之一.%Aim:To determine the serum levels of soluble intercellular adhesion molecule-l( sICAM-1 ) and leukocyternmembrane adhesion molecules( CD11a and CD18 ) in patients with congestive heart failure( CHF ). Methods:The serum specimens of eighty patients with CHF and fifty healthy controls were collected. The serum sICAM-1 was determined by ELISA.and CDlla and CD18 by flow cytometry. Results:The serum sICAM-1 level and the percent of CD11a+ or CD18+ cells of CHF patients were significantly higher than those of the control group( t = 10. 186,11. 999 ,and 13.764, P <0.05 ). Among the CHF patient caused by different heart disease,there were no significant differences in the serum sICAM-1 level and the percent of CD11a+ or CD18+ cells( F = 2. 578 ,1.723 ,0. 214,P >0. 05 ). There were significant differences in the serum sICAM-1 level,the percent of CD11a+ and CD18+ cells( F = 23. 953 ,15. 592,25. 384,P < 0. 001 ) among the CHF patients with different heart function degree, and those of IV degree CHF patients were higher than those of II or III degree patients( P <0.05 ). Conclusion:The inflammation reaction induced by ICAM-1 may be important for CHF.