瑞舒伐他汀对心肌梗死后大鼠心肌组织中α-SMA、 TGF-β1表达的影响

摘要

Aim:To observe the effects of rosuvastatin on the expressions of α-smooth muscle actin(α-SMA) and trans-forming growth factor-β1(TGF-β1) in rats after myocardial infarction and to discuss the influence mechanism of myocardial remodeling.Methods:The myocardial infarction model was induced by ligation of left anterior descending coronary artery .A total of 30 SD male rats with acute myocardial infarction were randomly divided into the myocardial infarction group and rosuv -astatin-treated group;another 15 rats were designed as sham-operated group .Rosuvastatin-treated group was given rosuvasta-tin at 1 mg/kg after operation,and the other 2 groups were given 2 mL normal saline each day.Four weeks later,the collagen synthesis,α-SMA,TGF-β1 expression were compared.Results:Collagen synthesis,α-SMA,TGF-β1 expression were reduced in the rosuvastatin-treated group compared with rats in the myocardial infarction group (P<0.05),and the 3 indexes in rosuv-astatin-treated group and myocardial infarction group were higher than those of the sham-operated group(P<0.05).Conclu-sion:Rosuvastatin could reduce the expression of α-SMA and TGF-β1 in the damaged myocardial tissue .%目的：通过观察瑞舒伐他汀对心肌梗死后大鼠心肌组织中α-平滑肌肌动蛋白（α-SMA ）和转化生长因子-β1（ TGF-β1）表达的影响，探讨其影响心肌重塑的机制。方法：45只SD雄性大鼠分为假手术组、心肌梗死组、干预组，每组各15只。假手术组只穿线不结扎，另外2组结扎冠状动脉左前降支建立心肌梗死模型。干预组术后给予瑞舒伐他汀钙1 mg／kg灌胃，心肌梗死组及假手术组每天以2 mL生理盐水灌胃，4周后比较各组大鼠心肌胶原容积分数、α-SMA、TGF-β1 mRNA和蛋白表达水平的差异。结果：给药4周后，与心肌梗死组相比，干预组心肌胶原容积分数及α-SMA、TGF-β1 mRNA和蛋白表达均降低（P均＜0．05）；干预组和心肌梗死组上述指标均高于假手术组（ P均＜0．05）。结论：瑞舒伐他汀能降低受损心肌中α-SMA、TGF-β1表达水平。