目的：探讨甘草次酸对哮喘大鼠肺组织NF-κB、IL-6、IL-5蛋白表达及抗氧化应激能力的影响。方法：采用卵清白蛋白致敏法制备大鼠支气管哮喘模型，并给予甘草次酸高、中、低（200、100、50 mg／kg ）剂量进行干预；同时设正常对照和地塞米松对照；每组10只大鼠。干预7 d后处死大鼠，用生化法测大鼠血清超氧化物歧化酶（SOD）、丙二醛（MDA）、总抗氧化能力（T-AOC）及谷胱甘肽过氧化物酶（GSH-Px），用RT-PCR和Western-blot法测大鼠肺组织NF-κB、IL-6及IL-5 mRNA及蛋白表达水平。结果：与正常对照比较，模型组和GA各剂量组大鼠血清SOD、GSH-Px及T-AOC水平降低，MDA水平升高，肺组织中NF-κB、IL-6及IL-5 mRNA和蛋白表达水平升高（ P＜0．05）。与模型组比较，甘草次酸高、中剂量组大鼠血清SOD、GSH-Px水平升高， MDA水平降低（P＜0．05），肺组织中NF-κB、IL-6及IL-5 mRNA和蛋白表达水平降低（P＜0．05），其中甘草次酸高剂量组变化更显著。结论：甘草次酸对哮喘大鼠氧化应激有拮抗作用，其机制与调控NF-κB信号通路有关。%Aim:To investigate the effects of glycyrrhetinic acid ( GA) on the NF-κB signal pathway and anti-oxidative stress capability in experimental asthma rats .Methods:Ovalbumin allergization was used to establish bronchial asthma rat model, and the model rats were given GA (200, 100, 50 mg/kg) through intragastric administration once a day for 7 days, respectively,blank control, asthma model control and dexamethasone control were employed in the experiment ,and 10 rats were in each group .The levels of SOD , MDA, GSH-Px and T-AOC in serum were determined by biochemical methods . The mRNA and protein expressions of NF-κB, IL-6 and IL-5 in lung tissue were determined by RT-PCR and Western blot , respectively.Results:Compared with the blank control group , the activity of SOD, GSH-Px and T-AOC in serum of asth-ma model control group and 3 GA groups decreased , the serum level of MDA was higher , and the mRNA and protein ex-pressions of NF-κB, IL-6 and IL-5 in lung tissue were higher(P<0.05).Compared with the asthma model control group , the activity of SOD and GSH-Px in serum of 200 and 100 mg/kg GA group were higher , the serum level of MDA was lower , the mRNA and protein expressions of NF-κB, IL-6 and IL-5 in lung tissue were lower(P<0.05),and the changes of 200 mg/kg GA group were more obvious .Conclusion:GA has the capability to against oxidative stress in asthma rats , which may be related to the NF-κB signal pathway .
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