4.1R 基因敲除小鼠血液生理生化参数分析

摘要

目的：检测并分析蛋白4．1R 基因敲除小鼠（4．1R－／－）和野生型小鼠（4．1R＋／＋）血液生理生化参数。方法：摘眼球法采集两组小鼠（n ＝6）血液，用全自动血液细胞分析仪和生化分析仪检测血液生理生化参数。结果：血液生理参数中，4．1R－／－小鼠红细胞计数、血红蛋白、红细胞压积和中性粒细胞比率显著低于野生型小鼠（P ＜0．05）；血小板计数、平均血小板体积、血小板压积、白细胞计数、淋巴细胞比率显著高于野生型小鼠（P ＜0．05）。生化参数中，4．1R－／－小鼠碱性磷酸酶显著低于野生型小鼠，总胆红素、直接胆红素和间接胆红素显著高于野生型小鼠（P ＜0．05）。结论：蛋白4．1R 缺失可导致慢性溶血性贫血，4．1R－／－小鼠相关血液生理生化参数随之改变。%Aim: To detect and analyze blood physiological and biochemical parameters of 4.1R gene knockout mice (4.1R-/-) and wild-type (4.1R+/+) mice.Methods: Blood samples of 4.1R-/- mice and wild-type mice were collect-ed by enucleating of eyeball , followed by blood physiological and biochemical parameters testing with automatic blood cell analyzer and biochemical analyzer .Results: Compared with those of the wild-type mice,RBC, HGB, HCT,and NEU of 4.1R-/- mice were obviously lower(P <0.05);while PLT,MPV,PCT,WBC and LYM of 4.1R-/- mice were obviously-/- mice were significantly higher(P <0.05).Conclusion: Deletion of protein 4.1R might result in chronic hemolytic anemia, and changes of blood physiological and biochemical parameters may be basic information for the further study with 4.1R higher(P <0.05).ALP,one of biochemical parameters , was significantly lower,while T-BIL, D-BIL and I-BIL of 4.1 R-/- mice model.