婴儿双歧杆菌对 ConA 诱导小鼠肝纤维化的影响

摘要

Aim:To research the preventive effects and mechanism of infant bifidobacteria on liver fibrosis of mice in -duced by concanavalin A(ConA).Methods: The mice were randomly allocated into three groups: normal group, model group and infant bifidobacteria-treated group,12 in each group.The ConA solution(12.5 mg/kg) was given by intravenous injection to the model group and infant bifidobacteria-treated group once a week , normal group and model group were given normal saline by gavage ,and infant bifidobacteria-treated group was given infant bifidobacteria (0.5 g/kg) by gavage once a day.After 7 weeks, liver weight index, liver function, concentrations of HA and PCⅢin serum, as well as collagen Ⅰ, collagen Ⅲ, MMP-1, MMP-2, MMR-9, TIMP-1,TNF-α, IL-6, IL-10 in liver tissue were determined .Results: Infant bifidobacteria could decrease liver weight index (P<0.05), improve the liver function(P<0.05), decrease the serum concentrations of HA and PC Ⅲ(P<0.05), decrease the contents of collagen Ⅰ, collagen Ⅲ, MMP-2, MMR-9, TIMP-1,TNF-α, IL-6(P<0.05) in mice liver tissue, and increase the contents of MMP-1 and IL-10 in mice liver tissue(P<0.05).Conclusion:Infant bifidobacteria could prevent liver fibrosis of mice induced by ConA .%目的：研究婴儿双歧杆菌对刀豆蛋白A（ ConA）诱导的小鼠肝纤维化的作用及其机制。方法：36只小鼠随机分为正常组、模型组和婴儿双歧杆菌组，每组12只。正常组静脉注射生理盐水，模型组和婴儿双歧杆菌组静脉注射12．5 mg／kg ConA溶液，每周1次。每日通过灌胃给予正常组和模型组蒸馏水，婴儿双歧杆菌组通过灌胃给予0．5 g／kg婴儿双歧杆菌溶液，持续7周。检测小鼠肝脏质量指数，小鼠血清肝功能生化指标（ALT、AST、ALB），透明质酸（ HA），Ⅲ型前胶原肽（ PC Ⅲ）的水平，以及小鼠肝组织Ⅰ型胶原（ ColⅠ）、Ⅲ型胶原（ Col Ⅲ）、基质金属蛋白酶抑制因子（ TIMP）-1、基质蛋白金属酶（ MMP）-1、MMP-2、MMP-9、肿瘤坏死因子α（ TNF-α）、IL-6、IL-10的含量。结果：婴儿双歧杆菌能显著降低ConA诱导小鼠的肝脏质量指数（P＜0．05），改善血清肝功能生化指标（P＜0．05），降低血清HA、PC Ⅲ水平（P＜0．05），降低肝组织中ColⅠ、Col Ⅲ、MMP-2、MMP-9、TIMP-1、TNF-α、IL-6含量（P＜0．05），升高小鼠肝组织中MMP-1、IL-10含量（P＜0．05）。结论：婴儿双歧杆菌具有明确的抗小鼠肝纤维化作用。