腺苷 A2 A受体对新生小鼠离体延髓脑片基本节律性呼吸放电的调节作用

摘要

Aim:To investigate the effect of adenosine A 2A receptor on basic rhythmic respiratory discharge activity ( RRDA) of medulla oblongata slice from neonatal mice .Methods: Medulla oblongata slices in vitro from neonatal mice were prepared, and then perfused with artificial cerebrospinal fluid (ACSF) which containing 0.0, 2.5, 5.0, 10.0, 20.0 nmol/L adenosine A2A receptor agonist CGS21680 or antagonist SCH58261 for 10 min.At the 6th min of perfusion, ad-sorption electrode was used to record the inhale duration (TI), discharge integral amplitude (IA) and respiratory cycle (RC).Results:CGS21680 could shorten RC, increase TI and IA(P<0.001), excite RRDA;SCH58261 could extend RC, reduce TI and IA(P<0.001),and has inhibitory effect on RRDA.Conclusion: The adenosine A2A receptor may play an excitatory role in RRDA of medulla oblongata in neonatal mice .%目的：探讨腺苷A2A受体对新生小鼠离体延髓脑片基本节律性呼吸放电（ RRDA）的调节作用。方法：制备新生小鼠离体延髓脑片，分别使用含0．0、2．5、5．0、10．0、20．0 nmol／L腺苷A2A受体激动剂CGS21680或拮抗剂SCH58261的人工脑脊液（ ACSF）灌流脑片，每个浓度灌流10 min，在每个浓度灌流第6 min时，使用吸附电极记录脑片RRDA，分析吸气时程（ TI）、放电积分幅度（ IA）和呼吸周期（ RC）。结果：CGS21680可以缩短RC，增加TI和IA（P＜0．001），对RRDA有兴奋作用。 SCH58261则可以延长RC，减小TI和IA（P＜0．001），对RRDA有抑制作用。结论：腺苷A2A受体参与调节新生小鼠延髓RRDA，激活腺苷A2A受体对RRDA有兴奋作用。