锯缘青蟹呼肠孤病毒宿主范围调查

摘要

锯缘青蟹呼肠孤病毒（Scylla serrata reovirus， SsRV）是锯缘青蟹养殖业中造成重大危害的传染病原之一。为了解SsRV的自然宿主范围，尽早发现和消除SsRV传染源，切断SsRV疾病传播途径，预防和控制SsRV疾病暴发流行，文章采用一步RT-PCR检测方法，对自然环境及青蟹养殖区的多种水生动物及其青蟹饵料携带的SsRV状况进行了检测。研究结果显示，多种甲壳动物、软体动物和青蟹饵料均携带SsRV病毒。研究结果表明， SsRV具有较广泛的宿主，揭示青蟹养殖过程中应该注意与这些海洋动物隔离，切断 SsRV传播途径，同时避免以带毒动物为饵料，消除传染源，减少SsRV病毒病的发生。%Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of the mud crab (S. serrata). To investigate the host range of SsRV, a number of marine species were collected in Ningbo, Zhejiang Province. The one-step RT-PCR was used to detect SsRV in these marine species. It was found that several crustaceans and molluscs were SsRV positive. Furthermore , some unclassified trash fish samples and Solen grandis as the diets of S.serrata were also SsRV positive. It was suggested that S. serrata should be cultured in the circumstances isolated from those marine species of SsRV positive so as to cut off the transmission route of SsRV and eliminate the origin of infection.