为了更好地开发利用条叶榕Ficus pandurata var.angustifolia这一畲族习用药用植物,以条叶榕无菌植株为外植体,对影响条叶榕叶片愈合组织诱导、不定芽分化、增殖和壮苗生根的主导因子植物生长调节物质进行了研究.结果表明:叶片在添加不同植物生长调节荆物质(包括6-苄基腺嘌呤6-BA,2,4-二氯苯氧乙酸2,4-D,萘乙酸NAA)和不同质量浓度的培养基中均能诱导愈合组织,且诱导率均达到100％,但愈合组织进一步分化成不定芽较为困难,以MS+1.0 mg·L-16-BA +0.2 mg·L-12,4-D +0.1 mg· L-1NAA分化率最高,为6.67％；不定芽在添加不同植物生长调节物质种类(苯基噻二唑基脲TDZ,6-BA,NAA)和不同质量浓度的培养基中均表现出较好的增殖效果,3种植物生长调节物质对不定芽增殖的作用大小依次为6-BA,TDZ和NAA,以MS+0.3 mg· L-1TDZ+1.0 mg·L-16-BA +0.3 mg·L-1NAA增殖倍数最高,达到8.93;条叶榕生根容易,生根率均达到100％.%Little information on callus induction,proliferation and regeneration from leaf explants of Ficus pandurata var.angustifolia,a commonly used medication with the She ethnic minority,has been found.To develop and utilize this ethnic herb,an in vitro induction and shoot regeneration from leaf explants was evaluated using an orthogonal experimental design with plant growth regulators,including 6-Benzylaminopurine (6-BA),2,4-dichlorophenoxyacetic acid (2,4-D),Thidiazuron (TDZ),and naphthaleneacetic acid (NAA),concentrations in a Murashige and Skoog (MS) media.Results showed that callus induction ratio in all 9 treatments exceeded 100％,but adventitious buds were extremely difficult to differentiate.The optimum medium for adventitious buds differentiation was MS + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 2,4-D + 0.1 mg·L-1 NAA,the differentiation rate of adventitious buds reached 6.7％.The orthogonal experimental design showed that the proliferation capacity of plant growth regulators was in the order:6-BA ＞ TDZ ＞NAA.The optimum medium for proliferation was MS + 0.3 mg·L-1 TDZ + 1.0 mg·L-1 6-BA + 0.3 mg·L-1 NAA,the multiplication rate was 8.93,and the rooting rate in all 9 treatments was 100％.The tissue culture technique and rapid propagation system of Ficus pandurata var.angustifolia could be used for large-scale seedlings in short time and provide technical guidance for large-scale production.
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