美人蕉和绿萝在修复富营养化水体过程中对Cr(Ⅵ)胁迫的生理生化响应

摘要

研究了陆生植物美人蕉(Canna indica)和绿萝(Scindapsus aureum)在修复富营养化水体过程中对不同质量浓度Cr(Ⅵ)胁迫的生理生化响应.结果表明,Cr(Ⅵ)胁迫影响植物生长,Cr(Ⅵ)质量浓度为10 mg·L-1时两种植物生长明显受到抑制.Cr(Ⅵ)质量浓度为2 mg·L-1时促进美人蕉及绿萝可溶性蛋白质的合成,提高了美人蕉及绿萝SOD、CAT、POD活性,对两种植物MDA积累无明显影响;当Cr(Ⅵ)质量浓度5 mg·L-1时抑制美人蕉和绿萝可溶性蛋白质合成及绿萝CAT活性,提高美人蕉SOD、CAT、POD及绿萝SOD、POD活性,对美人蕉MDA积累影响不大,增加绿萝MDA积累;当Cr(Ⅵ)质量浓度达到10 mg·L-1时,明显抑制两种植物可溶性蛋白质的合成,且抑制美人蕉CAT、POD活性和绿萝CAT活性,提高美人蕉和绿萝SOD活性及绿萝POD活性,显著增加两种植物MDA积累.本试验条件下,美人蕉生长速度大于绿萝,对铬胁迫的耐受能力更强.%The study on the physiological and biochemical responses of terrestrial plants(Canna indica and Scindapsus aureum) under Cr( Ⅵ ) stress in eutrophic water showed that Cr (Ⅵ) stress affected plant growth, Cr (Ⅵ) concentration of 10 mg· L-1 clearly limited the growth of the two kinds of plant. Cr( Ⅵ ) concentration of 2 mg · L-1 stimulated the soluble protein synthesis and SOD、CAT、POD activity of Canna inidca and Scindapsus aureum, had no significant effect on MDA accumulation of the two kinds of plants; 5 mg· L-1 Cr( Ⅵ ) inhibited the Canna inidca and Scindapsus aureum soluble protein synthesis and the Scindapsus aureum CAT activity, improved Canna inidca antioxidant enzymes activity and Scindapsus aureum SOD、POD activity, had no significant effect on MDA accumulation of Canna inidca, increased MDA accumulation of Scindapsus aureum; 10 mg· L-1 Cr(Ⅵ) treatment significantly inhibited the two kinds of plants soluble protein synthesis and Canna inidca CAT、POD activity and Scindapsus aureum CAT activity, increased SOD activity of two kinds of plants and Scindapsus aureum POD activity, significantly increased MDA accumulation of two kinds of plants. Under the experimental conditions, Canna inidca grew faster than Scindapsus aureum, were more tolerant to Cr(Ⅵ ) stress.