骨质疏松发病过程中骨髓间充质干细胞差异性表达microRNA的筛选及其在干细胞多向分化中的功能

摘要

Objective:To screen differentially expressed microRNAs in bone marrow- derived mesenchymal stem cells (BMMSCs) during osteoporosis and their function in stem cell differentiation. Methods; The osteoporosis model was induced by ovarectomy in mice. The differentially expressed microRNAs in BMMSCs during osteoporosis were screened with microRNA microarray chip and their expressions during osteogenesis and adipogenesis were detected by RT-PCR. Results: Ten differentially expressed microRNAs were detected in BMMSC during osteoporosis. The expression of 5 specificmicroRNAs altered significantly during osteogenic differentiation and 3 microRNAs altered during adipogenic differentiation. Conclusion: A serials of differentially expressed microRNAs may be involved in functional defects of BMMSC during osteoporosis.%目的:研究去势小鼠骨质疏松模型中骨髓间充质干细胞( BMMSC) microRNA表达谱的改变,确定特异性改变microRNA的功能,明确其在骨质疏松发病中的作用.方法:通过微阵列芯片筛查,寻找在骨质疏松模型骨髓间充质干细胞中差异性表达的microRNA,并在成骨成脂分化诱导后行RT-PCR检测其表达水平,研究其在BMMSC多向分化中的作用.结果:本研究发现了10个在骨质疏松发病过程中有差异性表达的microRNA,其中5个microRNA在BMMSC成骨分化过程中,3个microRNA在成脂分化过程中表达出现明显改变.结论:microRNA表达异常可能导致骨质疏松发病过程中BMMSC的功能缺陷.