四种不同产地白术的DNA随机扩增多态性研究

摘要

[Objective] To study the genetic diversity and provide the basis for the identification of Rhizoma Atractylodis Macroceph-alae [Methods] DNA fingerprint in four different populations of Rhizoma Atractylodis Macrocephalae was investigated by RAPD a-nalysis. Genetic distance and dendrogram of RAPD cluster among four populations was also analyzed by PopGen32 software. [Results] Fourteen primers were selected from total seventy primers to produce highly reproducible RAPD bands. A total of 215 bands with 138 polymorphism bands were amplified from four populations by fourteen effective primers. Ratio of genetic polymorphism was 64. 2%. A DNA molecular dendrogram was established by UPGMA analysis. [Conclusion]. High genetic diversity of genetic variation occurs in different populations of Rhizoma Atractylodis Macrocephalae. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of Rhizoma Atractylodis Macrocephalae.%[目的]分析不同产地白术的遗传多样性，为白术的种质鉴定提供依据。[方法]运用随机扩增多态性DNA(RAPD)技术，分析浙江、湖北、安徽、河南产白术的遗传多样性，利用PopGen32软件计算遗传距离，运用UPGMA法进行聚类分析并构建树状图。[结果]共筛选了70个随机引物，从中挑选出14条多态性强、重复性好的引物，总共检测出215个位点，多态性位点138个，多态位点比率为64.2％，UPGMA聚类可以将不同来源的白术很好地区分开来。[结论]不同产地间的白术存在丰富的遗传多样性，RAPD分子标记方法可以用来鉴定不同产地的白术。