[Objective] To observe the effect of Xiaoyu tongluo san on the second breast nipple diameter, height and E, level, ER expression of the Mammary Gland Hyperplasia rats model, discuss the therapeutic mechanism of HMG. [Methods] Divide 40 Wistar racs into 4 groups randomly: normal control group, model group, TCM group and western medicine group. Combined diethylstilbestrol with progesterone by intramuscular injection to copy breast Hyperplasia rats model. TCM group was treated with self-Xiaoyu tongluo san 50mg/d, while western medicine group was treated with TAM 1.8mg/Kg, gavage was both applied,1 time/d,30 days totally. Detect second breast nipple height, diameter, the levels of E2.ER expression and breast tissue pathological forms. [Results] Before and 10,20,30 days after treatment on companion of the second breast nipple diameter and height: after die success of made models, TCM group and western medicine group and model group were higher than the normal group(P<0.05);after treatment, TCM group and western medicine group were lower than model group(P<0.05);TCM group was lower than western medicine group. Comparison of E2 content: TCM group and western group were lower than model group; compared TCM group with western medicine group, the difference was not statistically significant Comparison of ER expression: TCM group and western group were higher than model group, TCM group was lower dian western medicine, group. Comparison of breast tissue pathological forms: The pathological forms in TCM group were similar to those in western group,but were both better than that in model group, and similar to the normal group. [Conclusion] The self—Xiaoyu tongluo san has effects on reducing nipple diameter, height and regulating the dysfuction of serum E2 and ER expression, and improve the breast tissue pathological forms of Mammary Gland Hyperplasia rac.%[目的]观察自拟消瘀通络散对乳腺增生大鼠乳头高度、直径的动态变化、血清E2、ER蛋白表达水平及病理组织形态的影响,探讨其治疗乳腺增生的作用机制.[方法]将40只Wistar大鼠随机分为正常组、模型组、中药组和西药组4组.采用己烯雌酚联合黄体酮肌注的方法进行造模.中药组予自拟消瘀通络散50mg/d,西药组予三苯氧胺1.8mg.kg-1,均灌胃治疗,1次/d,共30d.用精密游标卡尺于造模前后、治疗前、治疗10、20、30d分别测量大鼠第2对乳头高度和直径,测定血清E2水平及ER蛋白表达水平,取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现.[结果]治疗前及治疗10、20、30d4组大鼠第2对乳头直径、高度的比较:造模成功后,中药组、西药组、模型组均高于正常组(P＜0.05)；治疗10、20、30d后,模型组明显高于正常组(P＜0 05)；中药组和西药组均低于模型组(P＜0.05)；中药组低于西药组(P＜0.05)；中药组与正常组相比无显著性差异(P＞0.05).E2含量比较:中药组和西药组均低于模型组(P＜0.05),中药组和西药组比较无显著性差异(P＞0.05).ER蛋白表达水平比较:中药组和西药组均低于模型组(P＜0.05)；中药组低于西药组(P＜0.05).病理学比较:中药组和西药组大鼠乳腺组织的病理表现相似,与模型组比较改善明显,与正常组相近.[结论]自拟消瘀通络散具有缩小模型大鼠乳头直径、高度,降低血清中E2、ER蛋白表达水平和改善乳腺组织病理形态的作用.
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