消瘀通络散对乳腺增生大鼠乳腺组织形态与内分泌的影响

摘要

目的:观察自拟消瘀通络散对乳腺增生大鼠乳头直径、高度,血清E2/P、PRL、T水平以及乳腺组织形态的影响,探讨其治疗乳腺增生的作用机理.方法:将40只Wistar大鼠随机分为正常组、模型组、中药组和西药组4组.采用己烯雌酚联合黄体酮肌注的方法进行造模.中药组给予自拟消瘀通络散50mg/d,西药组予三苯氧胺1.8mg/kg,均灌胃治疗,1次/d,共30d.于治疗前后测量各组大鼠第2对乳头直径、高度,测定血清E2、P、PRL、T水平,计算E2/P数值,取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现.结果:治疗后乳头直径、高度比较,中药组和西药组均低于模型组(P＜0.05)；2组之间比较,中药组低于西药组(P＜0.05).血清各激素水平比较,中药组和西药组血清E2/P、PRL、T含量均低于模型组(P＜0.05)；中药组与西药组相比无显著性差异(P＞0.05).病理学比较,中药组和西药组大鼠乳腺组织病理表现相似,与模型组比较改善明显,与正常组相近.结论:自拟消瘀通络散具有缩小模型大鼠乳头直径、高度,改善内分泌紊乱状态和乳腺组织病理形态的作用.%Objective: To observe the effect of Xiaoyu tongluo san on the second pair of breast nipple diameter, height and serum E2/P ratio, PRL, T levels ^breast tissue of the Mammary Gland Hyperplasia rats model, discuss the therapeutic mechanism of HMG. Methods; 40 Wistar rats were divided into 4 groups randomly:normal group, model group, TCM group and western medicine group. Combined diethylstilbestrol with progesterone by intramuscular injection to copy breast Hyperplasia rats model. TCM group were treated with self-Xiaoyu tongluo san 50mg/d, while western medicine group were treated with TAM 1. 8mg/kg,gavage was both applied, 1 time/d, 30 days totally. Detected the second pair of breast nipple diameter ,height and serum E2/P ratio, PRL, T levels before and after treatment,calculate E2/P ratio, The second pair of nipple were removed aseptically, stained HE, breast forms was observed under microscope. Results; After treatment, comparision of the second breast nipple diameter and height; TCM group and western medicine group were lower than model group (P < 0. 05 ) ; TCM group were lower than western medicine group (P < 0. 05 ). Comparision of serum levels of the hormone; TCM group and western medicine group were lower than model group ( P < 0. 05 ) , compared TCM group with western medicine group, the difference was not statistically significant ( P > 0. 05). Comparision of breast tissue pathological forms; The pathological forms in TCM group were similar to those in TAM group, but they were both better than that in model group, and similar to the normal group. Conclusion; The self-Xiaoyu tongluo san has effects on reducing nipple diameter,height and regulating the dysfuction of serum E2/P ratio, PRL, T and improve the breast tissue pathological forms of Mammary Gland Hyperplasia rats.