中药牛大力抗疲劳抗应激作用的实验研究

摘要

Objective: To study the anti-fatigue and anti-stress function of Radix Millettiae Speciosae in Mice. Methods Established 7 Radix Millettiae Speciosae dose groups with low(5g·kg-1), medium(10g·kg-1) , high(20g·kg-1)and negative control group by the SPF male mice. The mice after adaptive raise for 3 days, the negative control group were given normal saline, and the Radix Millettiae Speciosae dose groups were given orally given aqueous extracts of 5,10,20g·kg-1 from Radix Millettiae Speciosae for 14 consecutive days. After the last dosed, the mice performed weight loading swimming test, hypoxia tolerance test, low temperature resistance test and high temperature resistance test, and statistics the average survival time of mice in these test. Results:Compared with control group, the Radix Millettiae Speciosae could significantly prolong the survival time in weight loading swimming test, hypoxia tolerance test, low temperature resistance test and high temperature resistance test(P<0.01), As the dose increased, the mice survival time was prolonged. Conclusion:The Radix Millettiae Speciosae has the effects of anti-fatigue and anti-stress.%目的：探讨中药牛大力的抗运动性疲劳和抗应激作用.方法：采用SPF级昆明雄性小鼠作为实验对象，设立牛大力低（5g·kg-1）、中（10g·kg-1)、高（20g·kg-1）剂量组和阴性对照组.适应性饲养7d后，每天固定时间内用牛大力水煎液给各剂量组小鼠灌胃，阴性对照组给予生理盐水，连续14 d.末次给药1h后分别对小鼠进行负重游泳试验、耐缺氧试验、耐低温试验以及耐高温试验，并统计各实验中小鼠的平均存活时间.结果：牛大力能够显著延长小鼠在负重游泳试验，耐缺氧试验，耐低温试验，耐高温试验中的存活时间(P＜0.01)；且随着牛大力水煎液浓度的增大，小鼠的存活时间也相对延长，呈剂量-反应关系.结论：牛大力具有一定的抗运动性疲劳和抗应激作用.