Cox-2在槲皮素抑制耐药白血病 HL-60、HL-60A 细胞增殖中的作用

摘要

Objective To investigate the inhibitory effect of Quercetin on leukemia HL-60 and HL-60A cell lines in vitro and the the role of Cox-2 plays during the reaction. Methods The CCK-8 assay was used to measure the inhibition of Quercetin on HL-60 and HL-60A cell lines,and a flow cytometry was used to detect the apoptosis of HL-60 and HL-60A cells after exposure to Quercetin;the expressions of Cox-2 in HL-60,HL-60A cells and in peripheral blood mononuclear cells (PMBC)isolated from healthy adults were detected by using Western-blot.The change tendency of Cox-2 expression in HL-60,HL-60A cells were detected before and after treatment with Quercetin at different concentrations. Results Quercetin inhibited HL-60 and HL-60A cell proliferation and induced apoptosis.Compared with healthy adult PBMC,low ex-pression in PBMC,the expression of Cox-2 on leukemia HL-60 and HL-60A cells increased,and the ex-pression of Cox-2 on HL-60A increased more significantly;the expression of Cox-2 on HL-60A decreased after the cells were exposed to Quercetin,the HL-60A cells decreased more significantly.Conclusion The ex-pression of Cox-2 was related to acute leukemia cells drug-resistance,Quercetin induced apoptosis by inhibi-ting the Cox-2 expression for inhibiting HL-60A cell proliferation.%目的：探讨槲皮素体外对急性白血病耐药细胞株 HL－60、HL－60A 的抑制作用及环氧化酶－2（Cox－2）在其中起的作用。方法CCK－8法检测槲皮素对 HL－60及 HL－60A 细胞的抑制作用，流式细胞仪检查槲皮素诱导细胞凋亡作用；Westert－blot 法检测 Cox－2在正常人外周血单个核细胞、HL－60及 HL－60A 细胞表达情况；观察不同浓度槲皮素处理 HL－60及 HL－60A 细胞前后 Cox－2表达的变化趋势。结果槲皮素可以诱导 HL－60及 HL－60A细胞凋亡及抑制其增殖；Cox－2在正常人外周血单个核细胞低表达，在 HL－60及 HL－60A 表达明显升高，HL－60A表达升高更加明显；经槲皮素处理后，Cox－2在 HL－60及 HL－60A 的表达明显下降，HL－60A 下降更加明显。结论Cox－2表达与急性白血病细胞耐药有关，槲皮素通过抑制 Cox－2的表达诱导 HL－60A 细胞凋亡而抑制细胞增殖。