Objective To establish a HPLC method for the determination of flavonoids content in caragana altaica Kom .Pojark by the studies on TLC identification characteristics of Caragana flowers in Aletai . Methods Quercetin and kaempferol were identified by TLC ,The HPLC analysis was performed on a Sun-fire C18 (150 mm × 4 .6 mm ,5 μm)column .a mixture of acetonitrile -0 .4% phosphoric acid aqueous solu-tion (50∶50) was used as the mobile phase with the flow rate at 1 mL/min .The detection wavelength was set at 360 nm .The column temperature was set at 25℃ .Results Quercetin and kaempferol could be de-tected by TLC quercetin ,kaempferol and isorhamnetin showed good linearity in the ranges of 0 .01 -0 .10 mg/mL ,0 .0025-0 .025 mg/mL and 0 .0025-0 .025 mg/mL .The average recoveries were 100 .7% , 100 .4% ,101 .7% .RSD1 .3% ,1 .4% and 1 .9% .Samples of flavonoid content averaged 1 .3% .Conclu-sion T he method is simple ,rapid ,accurate and reproducible and it can be used for the determination of the caragana altaica Kom .Pojark in the flavonoids content .%目的研究金雀花的薄层色谱鉴别特征，建立HPLC法测定黄酮类化合物的方法。方法采用薄层色谱法对金雀花中槲皮素和山萘酚进行定性鉴别，槲皮素以甲苯-乙酸乙酯-甲酸＝5∶2∶1为展开剂，山萘素以甲苯-乙酸乙酯-甲酸＝5∶4∶1为展开剂；采用色谱柱：Shim-pack VP-ODS（150 mm ×4．6 mm），以甲醇-0．4％磷酸溶液（50∶50）为流动相，流速为1 mL/min ，检测波长为360 nm ，柱温为25℃。结果在薄层色谱中能检测到槲皮素和山萘酚，槲皮素、山萘素、异鼠李素分别在0．01～0．1、0．0025～0．025、0．0025～0．025 mg/mL范围内线性良好，其平均回收率分别为100．7％、100．4％、101．7％。RSD分别为1．3％、1．4％和1．9％。样品黄酮类化合物的平均含量为1．25％。结论该方法简便、快速、准确、重复性好，可用于测定金雀花中黄酮类化合物的含量。
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