Study of Thickly and Thinly Sectioned Melanoma Skin Tissues with Mechanical Scanning Acoustic Reflection Microscopy.

摘要

The contents of the present study are organized into two parts: the first is focused on characterizing a thickly sectioned skin tissue with a mechanical scanning acoustic reflection microscopy (hereinafter simply called "SAM") of pulse-wave mode. The second part is focused on characterizing a thinly sectioned skin tissue with tone-burst-wave mode of SAM.;We showed analyses of optical and acoustical images, as well as developed mathematical models using an angular spectrum approach for predicting contrast and characterizing acoustic properties of both sections of tissue. Also, for thin specimens a reflectance function for the tissue located on a substrate was theoretically determined, and fitted into a mathematical model of the V(z) curve. Their leaky surface acoustic wave velocities were obtained from the experimentally formed V(z) curves through FFT analyses. Finally, a computer simulation with a parameter-fitting technique was implemented to obtain the longitudinal wave velocities and densities of the tissues.