异常黏液质证卵巢早衰及异常黑胆质证卵巢早衰模型建立及相关生殖指标比较

摘要

Objective To study the estrogen levels and biological characterization of the premature ovarian syndrome rat models in abnormal balgham and abnormal savda in Uygur medicine for the research refer-ence of the medical treatment for POF disorders.Method 30 Wistar female rats were selected and divided randomly into POF Group (NP)with unusual mucus,POF Group (HP)with unusual savda and normal control group (N),and their syndromes were compared and analyzed in terms of the related reproductive indicators.Results Unusual mucus quality and unusual savda led to POF and the major estrogen levels suggested the premature ovarian failure,with the indicators of decreased serum levels of estradiol (E2 ), increased follicle stimulating hormone (FSH)and luteinizing hormone (LH).The estrogen levels in NP group were more consistent with the syndromes of POF when compared with those in HP Group (P <0.05).There were no differences in HE staining and histological change in the ovarian pathology between NP/HP Group and N group.The weight of ovarian and adrenal glands were significantly greater than those of normal rats (P <0.05).Conclusion Abnormal mucus led to POF.%目的：研究维医异常黏液质与异常黑胆质型卵巢早衰（premature ovarian failure，POF）病证大鼠模型的雌激素水平与生物学表征，为维医治疗 POF 病症的研究提供依据。方法选用30只性功能正常的 Wistar 雌鼠，随机分为异常黏液质证 POF 组（NP）、异常黑胆质证 POF 组（HP）以及正常对照组（N），并分别建立异常黏液质证侯、异常黑胆质证侯，正常对照组正常环境饲养，对3组的相关生殖指标进行比较。结果异常黏液质、异常黑胆质均可导致 POF 发生，其主要雌激素水平检测提示均存在卵巢早衰，即血清雌二醇（E2）下降，促卵泡激素（FSH）及促黄体生成素（LH）上升，且与 HP 组大鼠雌激素变化水平相比，NP 组明显更加符合卵巢早衰（P ＜0．05）；两组症候 POF 组大鼠在 HE 染色观察卵巢病理学改变与正常组比较无明显差异，解剖后卵巢、肾上腺组织重量明显大于正常组大鼠，差异有统计学意义（P ＜0．05）。结论异常黏液质证更容易导致 POF。