Bmi-1、NANOG、EZH2在结直肠癌中的表达及临床意义

摘要

Objective To evaluate expression of Bmi-1,NANOG and EZH2 in tumor tissue in colorectal cancer patients and its relationship with tumor metastasis and lymph gland.Methods By using immuno-histochemical method,the expression of Bmi-1,NANOG and EZH2 in tumor tissue,the tissue adjacent to tumor and lymph gland was detected in 70 cases of colon and rectal cancer patients.Results Bmi-1, NANOG and EZH2 protein were of high expression in the tumor tissue.The positive expression rate of Bmi-1 was 81.4% (57/70)in the tumor tissue and 10.0% (7/70)in the tissue adjacent to tumor in the 70 cases of patients with colorectal cancer.The difference was statistically significant (χ2 =71.957,P <0.05). The positive expression rate of NANOG was 68.6% (48/70)in the tumor tissue and 15.7% (11/70)in the tissue adjacent to carcinoma tissues.The difference was statistically significant (χ2 =40.105,P <0.05);The positive expression rate of EZH2 was 85.7% (60/70)in tumor tissue and 22.9% (16/70)in the tissue adjacent to carcinoma tissues.The difference was statistically significant (χ2 =55.724,P <0.05).The ex-pressions of Bmi-1,NANOG and EZH2 were closely related to the clinical stage colorectal metastasis and postoperative survival (P <0.05),but have nothing to do with the patients gender,age and tumor size (P >0.05).There is no correlation between three proteins.Conclusion Three protein of Bmi-1,NANOG EZH2 may be valuable for the diagnosis of carcinoma.The expression of the three proteins is of guiding significance to colorectal cancer recurrence,metastasis and prognosis.%目的：探讨 Bmi-1、NANOG 和 EZH2蛋白在结直肠癌病人癌组织、癌旁正常组织和淋巴结中的表达及其与肿瘤患者临床病理的关系。方法应用免疫组化法检测70例结直肠癌病人癌组织、癌旁正常组织和淋巴结中 Bmi-1、NANOG 和 EZH2的表达情况，并分析其与临床病理的关系。结果Bmi-1、NANOG、EZH2蛋白均在肿瘤组织中高表达，70例结直癌患者中，Bmi-1在癌组织及癌旁组织中阳性表达率分别为81．4％（57／70）、10．0％（7／70），差异有统计学意义（χ2＝71．957，P ＜0．05）；NANOG 在癌组织及癌旁组织中阳性表达率分别为68．6％（48／70）、15．7％（11／70），差异有统计学意义（χ2＝40．105，P ＜0．05）；EZH2在癌组织及癌旁组织中阳性表达率分别为85．7％（60／70）、22．9％（16／70），差异有统计学意义（χ2＝55．724，P ＜0．05）；3种蛋白的表达均与结直肠癌的临床病理分期、淋巴结转移及术后生存期有关（P ＜0．05），而与患者性别、年龄及肿瘤大小无关（P ＞0．05）；3种蛋白的表达两两之间无相关性。结论Bmi-1、NANOG 与 EZH2蛋白对于结直肠癌的诊断及鉴别诊断有一定的意义；3种蛋白的表达对结直肠癌的术后复发、转移及其预后有指导意义。