维吾尔医异常黑胆质型哮喘与中医肾虚痰瘀型哮喘患者代谢组学特征的比较研究

摘要

目的：探讨维吾尔医异常黑胆质型哮喘与中医肾虚痰瘀型哮喘患者代谢组学特征的的异同。方法收集符合维吾尔医异常黑胆质证诊断标准的哮喘患者（异常黑胆质型哮喘患者，22例）符合中医肾虚痰瘀证诊断标准的哮喘患者（中医肾虚痰瘀型哮喘患者，35例）及健康志愿者（健康人，30例）的尿液样本，采用核磁共振波谱法（nuclear magnetic resonance，NMR）和高效液相色谱法（high-performance liquid chromatography，HPLC）检测代谢物相对和绝对含量；对各组尿液代谢物含量的差异性进行分析。结果 NMR研究结果显示，异常黑胆质型哮喘患者和肾虚痰瘀型哮喘患者尿液中β-羟丁酸、乳酸、丙酮、二甲胺、鲨肌醇和马尿酸等6种代谢物含量的变化相同，其中β-羟丁酸和马尿酸含量降低，而乳酸、丙酮、二甲胺和鲨肌醇4种代谢物含量升高，与健康人比较差异有统计学意义（P ＜0．05）。HPLC研究结果显示，异常黑胆质型哮喘患者和肾虚痰瘀型哮喘患者尿液中含量变化相似的氨基酸有天冬氨酸、苯丙氨酸、胱氨酸和精氨酸4种氨基酸；天冬氨酸、苯丙氨酸、胱氨酸和精氨酸在尿液中的含量明显降低，与健康人比较差异有统计学意义（P ＜0．05）。结论维吾尔医异常黑胆质型哮喘与中医肾虚痰瘀型哮喘患者体内代谢变化过程与方向基本相似。%Objective To investigate and analyze metabolic varieties and common characteristics of urine samples from asthma patients with Shenxu-Tanyu and abnormal Savda syndrome.Methods Urine samples from asthma patients with Shenxu-Tanyu syndrome,abnormal Savda syndrome and healthy con-trol group were analyzed by nuclear magnetic resonance (NMR)and high performance liquid chromatogra-phy (HPLC)spectroscopy.Results The NMR results show that,compared with the healthy people,the urine from patients with abnormal Savda and Shenxu-Tanyu syndrome had low level ofβ-hydroxybutyrate and hippuric acid,while the high level of lactic acid,acetone,dimethylamine and scyllo-inositol (P <0.05).The HPLC results showed that,the urine amino acid concentrations of asthma patients with Shenxu-Tanyu syndrome or abnormal Savda syndrome showed main significant differences compared with the healthy control group.The contents of aspartic acid,phenylalanine,cystine and arginine in urine for two kinds of syndromes were significantly decreased (P<0.05).Conclusion This results depicted that the asthma patients with different syndromes have very similar metabolic changes.