APE／Ref-1和Trx1在宫颈癌中的表达及相关性研究

摘要

Objective To explore the expression level of oxidation reduction factor-1 (APE/Ref-1)and thi-oredoxin 1 (Trx1 )in cervical cancer,To analysis the relationship between them and clinical pathology. Methods This experiment used Immunohistochemistry S-P method to detect the expression of APE/Ref-1 and Trx1 in 9 normal cervical tissue cases,20 cases of cervical intraepithelial neoplasia and 38 cervical cancer cases.And the results were analysed with relevant statistical methods.Results APE/Ref-1 and Trx1 had weak expression in normal cervical tissue (22.2%,12.5%),moderate expression in CIN (65.0%,55.0%)and strong positive expression in cervical cancer (84.2%,89.4%),respectively;The ex-pression of APE/Ref-1 was associated with the clinical stage,pathological classification of cervical cancer, while the expression of Trx1 was associated with the pathological classification and lymph nodes metastasis of cervical cancer.The expression of APE/Ref-1 and Trx1 protein were positively related in cervical cancer,and the difference was statistically significant (γ=0.557,P =0.000).Conclusion The expression of APE/Ref-1 and Trx1 protein were positively correlated in cervical cancer ,which may suggest that both of proteins may be involved in the occurrence and development of cervical cancer.%目的：探讨宫颈癌中氧化还原因子1（APE／Ref-1）和硫氧还蛋白1（Trx1）的表达及其与宫颈癌相关临床参数的关系。方法采用免疫组化 S-P法检测9例正常宫颈组织、20例宫颈上皮内瘤样病变（CIN）、38例宫颈癌中 APE／Ref-1和Trx1的表达水平，分析各自表达水平与宫颈癌相关临床参数的关系。结果在正常宫颈组织中 APE／Ref-1和Trx1均呈低表达，分别为22．2％、12．5％；在宫颈上皮内瘤变（CIN）中均呈中等量表达，分别为65．0％、55．0％；在宫颈癌组织中均呈高表达，分别为84．2％、89．4％；APE／Ref-1的表达与宫颈癌的临床分期、病理分级有关，Trx1的表达与宫颈癌的病理分级、淋巴结转移有关；在宫颈癌组织，APE／Ref-1和 Trx 1蛋白的表达呈正相关，差异有统计学意义（r＝0．557，P ＝0．000）。结论 APE／Ref-1和Trx1可能共同参与了宫颈癌的发生、发展。