体外静水压力作用下SB203580对大鼠髁突软骨细胞FAS蛋白和凋亡率的影响

摘要

Objective To observe the effect of SB203580 on the expression of FAS protein and apoptosis rate of mandibular condylar chondrocytes (MCCs) of rats under hydrostatic pressure in vitro,and to investigate the relationship between P38 channel and apoptosis of chondrocytes under the pressure.Methods MCCs were cultured in vitro and randomly divided into low dose group (10 μmol/L SB203580),high dose group (50 μmol/L SB203580) and control group,which all groups were given 30 kPa,12 h hydrostatic pressure.The expression of FAS protein was detected by Western Blot and the rate of apoptosis was tested by flow cytometry.Results The overall expression of FAS protein in the 3 groups was different (P ＜0.05),and FAS protein in the high dose group and low dose group were lower than that in the control group,and FAS protein in the high dose group was lower than that in the low-dose group with statistical significance (P ＜0.05).The apoptosis rate of the 3 groups were generally different,in which the high dose group and low dose group were lower than that in the control group,and that in the high dose group was lower than that in the low dose group with statistical significance (P ＜0.05).Conclusion SB203580,as an inhibitors of P38 channel could reduce the expression of FAS protein and the rate of apoptosis under hydrostatic pressure concentration-dependently,which is suggested that P38 signaling pathway is involved in the apoptosis of MCCs under pressure.%目的 观察静水压力作用下SB203580对大鼠髁突软骨细胞(mandibular condylar chondrocytes,MCCs) FAS蛋白表达量和凋亡率的影响,探讨P38通道与压力作用下软骨细胞凋亡的关系.方法 体外培养髁突软骨细胞,随机分为高剂量组、低剂量组和对照组.3组大鼠均给予30 kPa、12 h的静水压力;高剂量组和低剂量组分别给予高浓度(50μmol/L)和低浓度(10 μmol/L)的SB203580抑制剂进行干预处理.采用Western Blot方法检测FAS蛋白表达量,流式细胞仪检测细胞的凋亡率.结果 3组细胞中FAS蛋白表达量差异有统计学意义(P＜0.05),高剂量组、低剂量组FAS蛋白表达量均低于对照组,高剂量组低于低剂量组,差异有统计学意义(P＜0.05)D3组细胞的凋亡率差异有统计学意义(P＜0.05),其中高剂量组、低剂量组细胞凋亡率均低于对照组,高剂量组亦低于低剂量组,差异有统计学意义(P＜0.05).结论 P38信号通道抑制剂SB203580可抑制压力作用下髁突软骨细胞FAS蛋白的表达和细胞凋亡,且抑制效果与剂量浓度大小相关,提示P38信号通道参与了压力作用下髁突软骨细胞的凋亡过程.