目的：研究维吾尔族、汉族女性盆底器官脱垂（POP）患者阴道前壁结缔组织中弹性蛋白（Elastin）的表达及盆底支持组织结构和代谢的变化在 POP 发生、发展中的作用。方法选择新疆医科大学第一附属医院妇科收治的诊断为 POP、资料完整的 POP 患者118例（试验组），因子宫良性病变行全子宫切除术患者82例（对照组）。采用免疫组织化学方法，测定各组阴道前壁组织中 Elastin 在蛋白水平的表达。并采用逆转录-聚合酶链式反应（RT-PCR）法检测 Elastin 在基因水平的表达情况。结果Elastin 蛋白及 Elastin mRNA 在各组阴道前壁组织中均有表达，在试验组的表达量均明显少于对照组，差异有统计学意义（P ＜0．05），维吾尔族、汉族试验组之间及维吾尔族、汉族对照组之间 Elastin 蛋白及 Elastin mRNA 的表达差异均无统计学意义（P ＞0．05）。结论 Elastin蛋白对盆底各器官具有支持作用，其在盆腔器官脱垂疾病的发生、发展中发挥重要作用。Elastin 蛋白的表达无明显的维、汉种族差异性，此研究结果可为盆底功能障碍性疾病的研究提供一定的支持和帮助。%Objective To investigate the expression of Elastin in the onnective tissue of anterior wall of va-gina of both Uyghur and Han women patients with POP and to explore the change of pelvic support tissue structural and metabolic in the development and progression of POP.Methods Enrolled were 118 patients with POP having complete clinicopathological information and 82 patients undergone the hysterectomy ow-ing to benign uterine lesion.118 patients were assigned to experimental group whereas 82 cases were as control group.immunohistochemistry was used to assay the protein level of Elastin in the connective tissue of anterior wall of vagina.Reverse-transcriptase PCR (RT-PCR)was used to detect the mRNA level of E-lastin.Results Both mRNA and protein of Elastin were seen to be all expressed in the connective tissue of anterior wall of vagina.expression of Elastin was shown to be signifiantly decreased in experimental group than that of control group (P < 0.05);no sigfnicant difference was observed of expression of Elastin, whatever mRNA or protein level,between Han and Uyghur patients (P >0.05).Conclusion Elastin was proposed to be in support of pelvic organs,which may play an important role in the development and pro-gression of POP;Because of no difference of Elastin expression was observed between Han and Uyghur patients with POP,our findings may provide the favorable help and support for study on pelvic floor dysfunction disease.
展开▼
