The main purpose of this article was to designe a pair of specific primers which were amplified to Efb gene with 432 bp length by PCR,through isolating Staphylococcus aureus from Xinjiang bovine milk and collecting genome isolated from bacterial strain and referring Efb gene sequences published by Gen-Bank. The clone and sequence analysis of Efb gene were conducted on 9 strains of Staphylococcus aureus collected from 3 different areas in Xinjiang. The result showed that the amino acid homology of Efb protein from 3 areas in Xinjiang was 91. 0% -100%,compared with that of other areas,the amino acid homology of Efb protein was 86. 5%-100%,the difference among them was small. The data indicated that Efb was conserved.%对分离自新疆的奶牛乳源金黄色葡萄球菌,提取分离菌株的基因组DNA,参考GenBank中发表的细胞外纤维蛋白原结合蛋白(Efb)基因序列,设计特异性引物经PCR扩增得到长432 bp的Efb基因.对新疆三个不同地区分离的9株金黄色葡萄球菌分别进行Efb基因的克隆和序列分析,对Efb蛋白的氨基酸序列分析结果表明:新疆三个不同地区Efb蛋白氨基酸的同源性为91.0％～100％,与其他地区分离菌株的同源性为86.5％～100％,差异性较小.该结果表明,Efb基因在该菌进化和流行过程中是保守的.
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