糖基化神经酰胺合成酶和多药耐药相关蛋白在胰腺癌中的表达及其临床意义

摘要

Objective To study the expressions and clinical significance of glucosylceramide synthase (GCS) and multidrug-related protein (MRP) in pancreatic cancer. Methods The expressions of GCS and MRP were detected by immunohistochemistry in 30 pancreatic cancer cases and contrasted with 10 normal pancreatic tissues or pancreatitis, respectively. Results The positive staining of GCS was 60% in pancreatic cancer and 10% in normal pancreatic tissues or pancreatitis, with significant difference (P<0.05). The positive staining of MRP was 53.33% in pancreatic cancer and 10% in normal pancreatic tissues or pancreatitis, with significant difference (P<0.05). The positive expression of GCS and MRP did not show significant correlation with clinical findings including sex, age, tumor location, degree of differentiation, aggressiveness, lymph rodc mctastasis or TNM stage of tumors (P> 0.05). Conclusion The expressions of GCS and MRP are significantly higher in pancreatic cancer than in normal pancreatic tissues or pancreatitis. GCS and MRP may play an important role in multidrug resistance in pancreatic cancer, but their mechanisms are different. To examine GCS and MRP in clinic has an important guiding significance in determining chemotherapy strategy for pancreatic cancer.%目的 探讨糖基化神经酰胺合成酶(GCS)及多药耐药相关蛋白(MRP)在胰腺癌组织中的表达及其临床意义.方法 采用免疫组化SP法检测GCS、MRP在10例正常胰腺和胰腺炎及30例胰腺癌组织中的表达情况,并结合临床病理学特征进行研究.结果 GCS在胰腺癌组织中的阳性表达率为60％,在正常胰腺及胰腺炎组织中的阳性表达率为10％,胰腺癌GCS的阳性表达率显著高于正常胰腺和胰腺炎组织中的表达(P＜0.05)；MRP在胰腺癌中的阳性表达率为53.33％,在正常胰腺和胰腺炎中阳性表达率为10％,MRP在胰腺癌的阳性表达率显著高于在正常胰腺和胰腺炎组织中的表达(P＜0.05).GCS、MRP的表达与性别、年龄、原发肿瘤的位置、肿瘤的分化程度、肿瘤是否有侵袭性、是否有淋巴结转移以及肿瘤的分期之间差异无统计学意义(P＞0.05).结论 GCS、MRP在胰腺癌的多药耐药性中可能占有重要地位.其介导的耐药机制各不相同,临床检测GCS、MRP对胰腺癌化疗方案的制定具有重要的指导意义.