KCNJ11基因多态性与新疆哈萨克族原发性高血压的相关性

摘要

目的：探讨内向整流钾离子通道蛋白 J 亚单位11号成员(KCNJ11)基因多态性与新疆哈萨克族人群原发性高血压(essential hypertension,EH)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测 KCNJ11基因多态性位点 rs2285676的基因型,其中新疆哈萨克族高血压组(EH 组)126例,正常对照组(NT 组)126例。应用多因素非条件 Logistic 回归分析评价新疆哈萨克族高血压相关危险因素。结果Logistic 回归分析显示,KCNJ11-rs2285676位点基因型、性别、体质量、总胆固醇和甘油三酯与高血压无相关性；高密度脂蛋白(HDL)和体质量指数(BMI)是影响高血压的保护因素,低密度脂蛋白(LDL)水平是影响高血压的危险因素。KCNJ11多态性位点 rs2285676在高血压组中基因型 TT、CT、CC 及等位基因 T、C 频率分别为50.00%、48.41%、1.59%、74.21%和25.79%。高血压组与对照组差异无统计学意义(P >0.05)。结论KCNJ11基因 rs2285676多态性可能与新疆哈萨克族高血压无关。%Objective To investigate the relationship between the single nucleotide polymorphism (SNP)of KCNJ1 1 (inwardly rectifying potassium channel,subfamily J,member 1 1)gene and essential hypertension (EH)in Xinjiang Kazak population.Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP ) method was used to detect genotypes of rs2285676 polymorphism of KCNJ1 1, including 126 hypertensives (EH group)and 126 normotensives (NT group)in Xinjiang Kazak population.Multiple-factor unconditional Logistic regression analysis was used to evaluate the risk factors for hypertension in Xinjiang Kazak. Results Logistic regression analysis showed that KCNJ1 1-rs2285676 genotypes,sex,weight,total cholesterol and triglyceride were not associated with hypertension;body mass index (BMI)and high-density lipoprotein (HDL) were protective factors affecting hypertension while low-density lipoprotein (LDL ) was a risk factor for hypertension.Genotypes (TT,CT and CC)and allele frequencies (T and C)of the SNP of rs2285676 in KCNJ1 1 gene were 50.00%,48.41%,1.59%,74.21% and 25.79% in EH group,respectively.The differences between EH and normal control were not significant (P >0.05).Conclusion The rs2285676 polymorphism of KCNJ1 1 is not associated with EH in Xinjiang Kazak population.