miRNA-141、miRNA-145在非小细胞肺癌中的表达及与临床病理的关系

摘要

目的：通过检测 miRNA-141、miRNA-145在非小细胞肺癌中的表达,探讨其与非小细胞肺癌临床病理的关系。方法采用 Real time-PCR 法对48例非小细胞肺癌组织及正常肺组织的 miRNA-141、miRNA-145进行定量分析,结果由2(-△△CT)处理,并分析与临床病理资料的关系。结果①NSCLC 患者肿瘤组织中 miRNA-141表达水平明显升高,而且其表达与临床分期、病理类型显著相关(P <0.05),miRNA-141在鳞癌患者肿瘤组织表达高于腺癌(P <0.05),而在不同年龄、性别、肿瘤大小患者间差异无统计学意义(P >0.05)。②NSCLC 患者肿瘤组织中 miRNA-145表达水平明显下降,而且其表达与临床分期、病理类型显著相关(P <0.05),miRNA-145在腺癌患者肿瘤组织表达低于鳞癌(P <0.05),而在不同年龄、性别、肿瘤大小患者间差异无统计学意义(P >0.05)。结论miRNA-141高表达及 miRNA-145低表达与非小细胞肺癌的临床分期、病理分级密切相关,miRNA-141、miRNA145有可能作为非小细胞肺癌的重要肿瘤标志物。%Objective To detect the expressions of miRNA-141 and miRNA-145 in non-small cell lung cancer (NSCLC)and explore their relationship with the clinical pathology of NSCLC.Methods We made a quantitative analysis of miRNA-141 and miRNA-145 expressions in 48 cases of NSCLC tissues and adjacent normal tissues by real time-PCR.Then 2 (-△△CT) method was used to analyze their relationship with the clinicopathologic data.Results ① miRNA-141 expression was significantly increased in tumor tissue of NSCLC patients,and it also showed a significant correlation with clinical staging and pathological type (P <0.05).The expression of miRNA-141 in the tumor tissue in the patients with squamous carcinoma was higher than that in the patients with adenocarcinoma (P <0.05),but there was no significance difference in age,gender,tumor size or smoking history of the patients (P >0.05).② miRNA-145 expression was significantly decreased in the tumor tissue of NSCLC patients,and it also had a significant correlation with clinical staging and pathological type (P <0.05).The expression of miRNA-141 in the tumor tissue of the patients with adenocarcinoma was lower than that in the patients with squamous carcinoma (P <0.05).However,no significance difference was found in age,gender,tumor size or smoking history of the patients (P >0.05).Conclusion High expression of miRNA-141 and low expression of miRNA-145 are closely related to clinical staging and pathological type of NSCLC.miRNA-141 and miRNA-145 have the potential to be used as one of valuable tumor markers of NSCLC.