TNF-α基因-308位点多态性与新疆维吾尔族、哈萨克族人群中支气管哮喘的关系

摘要

Objective To investigate the relationship between the polymorphism of the tumor necrosis factor-αgene-308 promoter and bronchial asthma (BA)in Uighur and Kazakh population in Xinjiang.Methods PCR product sequencing method was used to detect the polymorphism distribution of TNF-αgene in 60 BA patients and 60 controls among Xinjiang Uighur and Kazakh population.Then we analyzed the association between different TNF-αgenotypes and BA in Uighur and Kazakh population.Results The distribution of GG,GA and AA genotype was significantly different between the two groups.Statistical analysis showed that BA group had a significantly higher TNF-αA allele frequency than that of the control one (P <0.05).After adjustment for sex and age,we found the A allele was a risk factor for BA pathogenesis (P <0.05).Conclusion The polymorphism of TNF-α gene-308 may be associated with the susceptibility to BA in Xinjiang Uighur and Kazakh population,so TNF-α gene-308 may be considered as a genetic marker for early identification of individuals at high risk for BA,which may play an important role in preventing the development of BA in clinical practice.%目的：探讨 TNF-α基因-308位点多态性与新疆维吾尔族、哈萨克族人群中支气管哮喘(BA)的相关性。方法以新疆维吾尔族、哈萨克族人群中 BA 发病组和对照组均60例为研究对象，通过 PCR 产物直接测序法检测 TNF-α基因-308位点的多态性分布，并统计分析 TNF-α不同基因型与新疆维吾尔族、哈萨克族人群中 BA 的相关性。结果维吾尔族、哈萨克族的 BA 发病组和对照组中均以 GG 基因型为主，在两民族人群中 GG、GA、AA 三种基因型频率有统计学差异(P <0.05)，等位基因 G 和 A 之间亦有统计学差异(P <0.05)；调整年龄、性别等影响因素后，两组之间仍有统计学差异(P <0.05)。结论TNF-α基因-308位点的多态性与新疆维吾尔族、哈萨克族 BA 的易感性相关，携带AA 或 GA 基因型患者发病风险较高。