胆囊癌组织中 Cyr61和β-catenin的表达及临床意义

摘要

Objective To evaluate the expressions of Cyr61 and β‐catenin protein in gallbladder carcinoma tissues and investigate their association with the clinicopathologic features of gallbladder carcinoma patients . Methods The expressions of Cyr61 and β‐catenin protein in 50 cases of gallbladder carcinoma and 19 cases of normal tissue were detected by immunohistochemical S‐P method .Results ① The positive expression rate of Cyr61 in gallbladder carcinoma tissues was 66 .0% (33/50) ,which was significantly higher than that in the normal tissues group (26 .3% ) .The expression of Cyr61 was related to tumor differentiation ,TNM stage and lymph node metastasis of gallbladder carcinoma (P=0 .010 ,P=0 .014 ,P=0 .007;P<0 .05) .② The positive expression rate ofβ‐catenin in gallbladder carcinoma tissues was 84 .0% (42/50) ,which was significantly higher than that in the normal tissues group 15 .7% (3/19);the expression of β‐catenin was related to tumor differentiation ,TNM stage and lymph node metastasis of gallbladder carcinoma (P=0 .018 ,P=0 .002 ,P=0 .024;P<0 .05) .③ Correlation test showed that Cyr61 andβ‐catenin were positively correlated in gallbladder carcinoma and adjacent normal tissues (r=0 .378 , P< 0 .05) .Conclusion Cyr61 and β‐catenin are highly expressed in gallbladder carcinoma tissues . Cyr61 andβ‐catenin expressions are closely related to the clinicopathologic features (tumor differentiation ,TNM staging and lymph node metastasis) in gallbladder carcinoma .Cyr61 and β‐catenin may have a synergistic effect in promoting progression and development of gallbladder carcinoma .Combined detection of Cyr61 and β‐catenin in gallbladder carcinoma tissues will contribute to the clinical diagnosis and prognosis .%目的：研究Cyr61、β‐catenin在胆囊癌组织及癌旁正常组织中的表达差异及相关性，探讨其在胆囊癌发生发展过程中的作用。方法应用免疫组织化学SP法，检测50例胆囊癌组织及19例癌旁正常组织中Cyr61和β‐catenin蛋白的表达，并与其临床病理特征进行比较分析。结果①Cyr61在胆囊癌组织中的阳性表达率为66．0％（33／50），明显高于正常组织中的26．3％（5／19）（P＜0．05）；Cyr61的表达与胆囊癌的肿瘤分化程度、TNM 分期、淋巴结转移有关（P＝0．010、P＝0．014、P＝0．007，P＜0．05）；②β‐catenin在胆囊癌组织中的阳性表达率为84．0％（42／50），明显高于正常组织中的15．7％（3／19）（P＜0．05）；β‐catenin的表达与胆囊癌的肿瘤分化程度、TNM 分期、淋巴结转移有关（P＝0．018、P＝0．002、P＝0．024，P＜0．05）；③相关性检验显示，Cyr61和β‐catenin在胆囊癌组织和癌旁正常组织中呈正相关（r＝0．378，P＜0．05）。结论 Cyr61和β‐catenin在胆囊癌组织中存在显著高水平表达；Cyr61和β‐catenin阳性表达与胆囊癌患者的临床病理特征（肿瘤分化程度、TNM分期和淋巴结转移）密切相关；两者在胆囊癌的发生发展过程中可能有协同促进作用，联合检测这两种蛋白的表达对胆囊癌的临床诊断及预后判断有一定的指导意义。