Experimental Study of Porous Beta-tricalcium Phosphate and Bone Morphogenetic Protein/porous Beta-tricalcium Phosphate Complex in Rabbit Mandibular Reconstruction

摘要

We investigated the osteogenic characteristic and biodegradation behavior of porous beta-tricalcium phosphate(β-TCP) and bone morphogenetic protein/beta-tricalcium phosphate(BMP/β-TCP) complex in mandibular reconstruction and evaluated the advantages of BMP/β-TCP complex in repairing bone defects. Bone defects created in the lower margin of bilateral mandible bodies in 12 rabbits were repaired with β-TCP (control group) and BMP/β-TCP complex (experimental group) respectively. The rabbits were euthanized after 2, 4, 8 and 12 weeks and examined by macroscopy, radiography, histology, histomorphometry and image analysis. 2 weeks after surgery, ossification of newly-generated tissue in BMP/β-TCP complex group appeared and after 12 weeks, massive new bone and ossification maturation were seen. However, in β-TCP group without BMP, ossification was not found until 12 weeks after operation. Image analysis showed that bone regeneration rate of BMP/β-TCP was 30%-40% higher and the degradation rate was 20%-30% higher than that of β-TCP. Therefore, as a reconstructive material for bone defects, BMP/β-TCP complex is superior to β-TCP and can be used in oral and maxillofacial surgery.