Activities of the Deriviatives of Chitin on the Osbeoblast Proliferation and the Effect on Bone Strength in Ovariectomized Rats

摘要

This study intends to examine the effects of different concentrations of four kinds of degradations of chitin: glucosamine (GLC),N-acetyl-D-glucosamine (NAG), chitooligosaccharide(COS),CM-chitooligosaccharide (CM-COS) —on the proliferation of MC3T3-E1 cell line cultured in vitro. Results suggest that all of the glucoses mentioned above promoted the proliferation of osteoblast, and various concentrations have different effects: the proliferation was remarkable when the concentration of GLC, NAG, COS, CM-COS was 100 , 100 , 500 , 500 μg/mL ,respectively. Furthermore we choose the glucosamine as the material and study the effect on the bone strength in ovariectomized rats. The results showed that the middle does of glucosamine can significantly increase the bone strength of femur in ovariectomized rats.