Anti-HIV-1 Activities of 4 Telomerase Restrictors

摘要

MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum tox-icity to the selected cells. FACSort flow cytometer and Innotest P24 HIV(Human immunodeficiency Virus) antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Pe-ripheral Blood mononuclear cells) and CEM/GFP if the concentra-tion of TRs was at 50 μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infec-tivity, which suggested that the TRs may be one of the novel re-sources for screening anti-HIV-1 agents.