青蒿内生真菌分离、分子鉴定及深绿木霉对青蒿的促生作用研究

摘要

为从青蒿(Artemisia annua L.)内生真菌中筛选有促生作用的菌株,用组织分离法从青蒿侧枝中分离内生真菌并进行分子鉴定,对其中1株真菌深绿木霉对青蒿幼苗的促生作用进行了研究.结果表明,从青蒿侧枝中获得23株内生真菌,鉴定出16种,以炭疽菌属占优势.深绿木霉发酵液对青蒿种子发芽和幼苗生长具有显著抑制作用;深绿木霉液体发酵菌丝体在4-5月对青蒿幼苗生长具有显著的促进作用,但7月以后没有明显促进作用.因此,深绿木霉可作为青蒿苗肥,与化肥配合可施用于成株.%In order to screen fungi with promoting effect on Artemisia annua growth,the endophytic fungi were isolated from A.annua brunches by tissue isolation method and molecular identified.The promoting effect of Trichoderma atroviride was studied.The results showed that 23 endophytic fungi were isolated belonging to 16 speices,in which the genus Colletotrichum was dominant.The fermentation broth of T.atroviride had significantly negative effect on seed germination and seedling growth of A.annua The mycelium of T.atroviride liquid fermentation had significant promoting effect on seedling growth of A.annua during April to May,but did not after July.So,it was suggested that T.atroviride could be used as fertilizer for seedlings of A.annua,and it combining with chemical fertilizer could also be applied to adult plants.