This study investigated the expression levels of insulin-related peptide receptor gene (irr) and several shell matrix protein genes (nacrein, efcbp, nl9, aspein, and accbp) in human recombinant growth hormone injection and insulin-like growth factor injection groups compared to the reference group in pearl oyster Pinctada fucata Gould. The expression level of irr was increased by hormone treatments (P<0.05), which is consistent with its role as insulin-like growth factor receptor in mollusk. Higher transcripts of nacrein indicated higher growth metabolism levels in the pearl oysters of the experimental groups; compared to the reference group, the expression levels of n19, aspein and accbp decreased after growth hormone (GH) and insulin-like growth factor- I (IGF- I ) treatments (P<0.05), suggesting they were suppressed by the GH and IGF- I pathways. In addition, the expression levels of aspein and accbp were found to be highly correlated; we infer that these two genes may be regulated by a single upstream factor. The expression level of efcbp showed a relatively steady expression in all oysters, and no significant differences were found among these groups (P>0.05).%研究了外源生长激素与胰岛素样生长因子-Ⅰ处理对马氏珠母贝Pinctada fucata Gould胰岛素相关多肽受体基因(irr)和5种壳基质蛋白基因(nacrein、efcbp、n19、aspein及accbp)表达水平的影响.结果表明,激素处理显著提高了irr基因的表达水平(P＜0.05),这与其在软体动物中作为胰岛素样生长因子受体的作用相一致.nacrein 基因的表达水平在激素处理组也有显著升高(P＜0.05),表明生长激素与胰岛素样生长因子-Ⅰ两种外源激素都增强了马氏珠母贝的生长代谢水平；与对照组相比,n19、aspein与accbp3个基因的表达水平在激素处理组均下调(P.＜0.05),说明这3个基因的表达受到激素调节通路的抑制作用.此外,研究发现aspein与accbp两个基因的表达在各个实验样本中具有极高的相关性,说明这两个基因在激素通路中可能受到同一个上游因子调控.efcbp基因表达水平在激素处理组与对照组之间表达稳定,各样本之间无显著性的变化(P＞0.05).
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