目的 建立基于可溶性童虫抗原(SSA)早期诊断日本血吸虫病的IHA法.方法 收集日本血吸虫感染后第13d童虫,制备SSA;探索SSA致敏红细胞的最佳体积比例以及灭活补体阴性兔血清的最佳封闭浓度,制备基于SSA的IHA法诊断试剂(以下简称"SSA-IHA法"),并检测日本血吸虫感染前及感染后第1、2、3、4、5、6周兔血清.结果 SSA致敏红细胞的最佳体积比例为1:1,灭活补体阴性兔血清的最佳封闭浓度(V/V)为3%.SSA-IHA法检测日本血吸虫感染前及感染后第1、2、3、4、5、6周兔血清的阳性率依次为0%、0%、0%、90.91%、100%、100%和100%.结论 基于可溶性童虫抗原(SSA)的IHA法在早期诊断日本血吸虫病方面具有一定的价值.%Objective To develop an IHA method by using soluble schistosomula antigen (SSA) for ear-ly diagnosis of schistosomiasis japonica. Methods Schistosomula were collected 13 days post-infection and SSA were prepared. The optimal volume ratio between SSA and erythrocyte and the best blocking concentra-tion (V/V) by negative rabbit sera of inactivating complement were explored. Then the IHA method based on SSA (SSA-IHA) was constructed and the sera from non-infected rabbits and 1,2,3,4,5,6 weeks post-infec-tion were detected. Results The optimal volume ratio between SSA and erythrocyte was 1:1 and the best blocking concentration (V/V) by negative rabbit sera of inactivating complement was 3%. The positive rate of sera from non-infected rabbits and 1,2,3,4,5,6 weeks post-infection were 0%,0%,0%,90.91%,100%,100%,and 100%,respectively when tested by SSA-IHA. Conclusion The IHA method based on SSA has val-ue on early diagnosis of schistosomiasis japonica.
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