目的研究肿瘤坏死因子-α（ TNF-α）与声门不同状态下闭合性胸部撞击所致肺损伤的关系。方法采用逆转录聚合酶链反应（ RT-PCR）法检测48只新西兰大白兔声门紧闭与（或）开放状态下，不同时相点伤侧肺组织TNF-αmRNA的表达，以6只正常新西兰大白兔为对照。结果在伤后2h时，声门紧闭与开放两组TNF-αmRNA的表达无显著差别（P＞0.05），伤后4、8、12h声门紧闭组TNF-αmRNA的表达显著高于声门开放组（ P＜0.05）。伤侧肺组织中TNF-αmRNA表达各时相点显著高于对照组（ P＜0.05）。结论TNF-α参与创伤性肺损伤的发生发展并起重要作用，声门紧闭状态下肺撞击伤的损伤程度重于声门开放状态。%Objective To study the relationship between TNF-αand lung injury induced by blunt chest trauma under different glottis states .Methods The TNF-αmRNA expression of the injured lung tissue in the 48 New Zealand rabbits was detected by reverse transcription polymerase chain reaction ( RT-PCR) under the closed and open glottis states at designed time .Meanwhile,6 healthy New Zealand rats were taken as control .Results There was no remarkable difference in the expressions of TNF-αof lung tissue between the closed glottis group and the open glottis group after 2 hours after injury(P>0.05).But the TNF-αmRNA expression was obviously higher in the closed glottis group than that in open glttis group at 4,8,and 12 hours after injury(P<0.05).TNF-αmRNA expressions were much higher in injured lung tissue than that in control group at designed time (P<0.05).Con-clusion TNF-αis involved in the occurrence and development of traumatic lung injuries and plays a critical role in this process .The severity of lung injuries under the closed glottis state is significantly higher than that under open glottis state .
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