Efficient oil biodegradation bacteria have been screened out using permutation and combination methods.Then preparations for free and immobilized agents,bioremediation tests of simulated petroleum-contaminated soils in lab,and GC-MS analysis of petroleum components before and after degradation were carried out,respectively.The results showed that combination 10#,mixed with a volume ratio of seed concentrate of 10-1 to 10-2,and 10-3 as 1﹕1﹕1 in shake-flask cul-tures,had the highest degradation rate of 73.34%, and no paraffin component was detection except for C18.At a low dos-age(10%)indoors for 60 d,immobilized agents of 10# showed a higher degradation rate(32.5%)than that of thefree agents(24.9%),and its short-paraffin content(11.42%)was also higher than that of both the free agents(7.91%)and the con-trol group(5.12%). This study provides foundations for the application of immobilized 10# agents to petroleum-contaminated soils.%为生物修复石油污染的土壤，采用排列组合法以石油降解率为指标筛选出高效石油降解菌群，制备游离及固定化菌剂，室内模拟石油污染的土壤并进行生物修复实验，采用气相–质谱联用(GC-MS)技术分析降解前后石油成分的变化．结果表明：摇瓶培养条件下，由菌株10-1、10-2和10-3的种子浓缩液按体积比1﹕1﹕1的比例组成的10#菌群石油降解率最高(73.34%)，且降解后除 C18组分外，其他烷烃成分未被检出；在10%,的投菌量下，60,d 后，10#菌群固定化菌剂组对室内石油污染土壤的石油降解率(32.5%)高于游离菌剂组(24.9%)，且降解后其短链烷烃含量(11.42%)也高于游离菌剂组(7.91%)和空白组(5.12%)．本文研究结果为10#菌群应用于野外石油污染土壤的修复奠定了基础．
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